Abstract After IEF-IPG (see Chapters 21–24), the IPG gel strips are equilibrated in the
presence of SDS, DTT, urea, glycerol, and iodoacetamide, and placed onto the surface of a …

Abstract Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

One-dimensional separations have limited resolving power. When two different
polyacrylamide gel electrophoresis techniques are combined, resolution can be enhanced …

Special problems require special solutions. That is why, despite the trend towards
standardization of the procedures in proteomic investigations, a number of alternative …

Summary Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry is currently the workhorse for the …

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of
proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and …

In proteomics analyses, gel-based approaches, including twodimensional gel
electrophoresis (2DE) and differential in-gel electrophoresis (DIGE), and gel-free mass …

D gel electrophoresis is one of the most effective techniques for high-resolution separation
of complex protein mixtures. Recent developments in the field of protein mass spectrometry …

The current laboratory manual of two-dimensional electrophoresis with immobilized pH
gradients (IPG-Dalt) was originally prepared for our own use and for the use by those who …

First devised in a time dominated by one-dimensional SDS-PAGE, immunoblotting (also
dubbed “Western” blotting,[1, 2]), is now widely used in conjunction with 2-D PAGE …