Methodologies to reprogram somatic cells into patient-specific pluripotent cells, which could
potentially be used in personalized drug discovery and cell replacement therapies, are …

To fully understand self-renewal and pluripotency and their regulation in human embryonic
stem cells (hESCs), it is necessary to generate genetically modified cells and analyze the …

Pluripotent embryonic stem cells (ESCs) maintain self-renewal while ensuring a rapid
response to differentiation cues. The identification of genes maintaining ESC identity is …

OCT4 is a crucial transcription factor in the pluripotent stem cell gene regulatory network and
an essential factor for pluripotent reprogramming. We engineered the previously reported …

OCT4A is well established as a master transcription factor for pluripotent stem cell (PSC) self-
renewal and a pioneer factor for initiating somatic cell reprogramming, yet its presence and …

Our knowledge of the molecular mechanisms underlying human embryonic stem cell
(hESC) self-renewal and differentiation is incomplete. The level of octamer-binding …

Embryonic Stem Cells (ES cells) exhibit vast potential that is just beginning to be realized
with regard to both clinical therapies, and use in the laboratory as a model system for the …

While most factors used as reprogramming transgenes can be replaced by other means,
Oct4 has remained essential until now. Three recent papers have now broken this barrier …

Oct4 has been implicated in regulation of pluripotency in embryonic stem cells (ESCs) and
reprogramming of somatic cells into induced pluripotent stem cells. However, the molecular …

Background Methods based on site-specific recombinases are widely used in studying gene
activities in vivo and in vitro. In these studies, constitutively active or inducible variants of …