One of the well-established approaches for the quantitative characterization of large-scale
underdetermined metabolic network is constraint-based flux analysis, which quantifies …

Abstract 13C‐Metabolic Flux Analysis (13C‐MFA) and Flux Balance Analysis (FBA) are
widely used to investigate the operation of biochemical networks in both biological and …

13C-constrained flux balancing analysis based on gas chromatography–mass spectrometry
data is presented here as a simple and robust method for the estimation of intracellular …

The field of metabolic engineering is primarily concerned with improving the biological
production of value-added chemicals, fuels and pharmaceuticals through the design …

Background Stoichiometric models constitute the basic framework for fluxome quantification
in the realm of metabolic engineering. A recurrent bottleneck, however, is the establishment …

A novel approach to 13C metabolic flux analysis (MFA) is presented using cytosolic
metabolite pool sizes and their 13C labeling data from an isotopically non-stationary 13C …

We have developed a novel approach for measuring highly accurate and precise metabolic
fluxes in living cells, termed COMPLETE-MFA, short for complementary parallel labeling …

Background The importance and power of isotope-based metabolic flux analysis and its
contribution to understanding the metabolic network is increasingly recognized. Its …

Traditionally, the intracellular fluxes of complex metabolic networks were quantified by
isotopic-tracer experiments, but, owing to practical limitations,'metabolic-flux balancing'is …

Constraint‐based modeling methods, such as Flux Balance Analysis (FBA), have been
extensively used to decipher complex, information rich‐omics datasets to elicit system‐wide …