Developments in microanalytical methods are enabling quantitative measurement of
multiple metabolic fluxes and, in conjunction with transcript and proteomic profiling, are …

Quantitative assessment of single cell fluxome is critical for understanding the metabolic
heterogeneity in diseases. Unfortunately, laboratory-based single cell fluxomics is currently …

Background The importance and power of isotope-based metabolic flux analysis and its
contribution to understanding the metabolic network is increasingly recognized. Its …

Abstract Accelerating the Design–Build–Test–Learn (DBTL) cycle in synthetic biology is
critical to achieving rapid and facile bioengineering of organisms for the production of, eg …

13C-metabolic flux analysis (13C-MFA) allows metabolic fluxes to be quantified in living
organisms and is a major tool in biotechnology and systems biology. Current 13C-MFA …

Highlights•The metabolic state of a cell is an important determinant of cellular
phenotype.•Current methods for global metabolic flux quantification characterize bulk …

Abstract Background Flux Balance Analysis is a theoretically elegant, computationally
efficient, genome-scale approach to predicting biochemical reaction fluxes. Yet FBA models …

Quantitative and qualitative knowledge of metabolic rates (ie fluxes) over a metabolic
network and in specific cellular compartments gives insights into the regulation of …

Metabolic flux analysis (MFA) methods use external flux and isotopic measurements to
quantify the magnitude of metabolic flows in metabolic networks. A key question in this …

Many technologies have been developed to help explain the phenotypic consequences of
genetic and/or environmental modifications in areas like functional genomics …