Despite the unshakable status of reverse transcription-quantitative PCR in gene expression
analysis, it has certain disadvantages, including that the results are highly dependent on the …

Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive
technique for gene expression analysis, depends on the stability of the reference genes …

Real-time quantitative polymerase chain reaction (Real-Time PCR) is a rapid, highly
sensitive, and highly specific technique, which is widely used to determine the relative …

Reverse transcription quantitative real‐time polymerase chain reaction (RT‐qPCR) is an
accurate and convenient method for mRNA quantification. Selection of optimal reference …

Appropriate choice of reference genes for data normalization is of critical importance for
accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene …

Key message This study proposed the combination of PR26S and PP1 as a good choice for
RT-qPCR normalization in pecan under abiotic stress, developing kernels, grafting, and …

Reverse transcription quantitative real-time PCR (RT-qPCR) has become one of the most
widely used methods for gene expression analysis, but its successful application depends …

Despite its superiority for evaluating gene expression, real-time quantitative polymerase
chain reaction (qPCR) results can be significantly biased by the use of inappropriate …

The success of quantitative real-time reverse transcription polymerase chain reaction (RT-
qPCR) to quantify gene expression depends on the stability of the reference genes used for …

Real-time quantitative PCR (RT-qPCR) has a high sensitivity and strong specificity, and is
widely used in the analysis of gene expression. Selecting appropriate internal reference …