Gene expression patterns of a target gene in different tissues and at different stages of
development can provide clues towards the understanding of its biological function. The …

Radish (Raphanus sativus) is an important cruciferous root crop with a close relationship to
Chinese cabbage (Brassica rapa). RT-qPCR is used extensively to evaluate the expression …

Selection of reference genes in Brassica napus, a tetraploid (4×) species, is a very difficult
task without information on genome and transcriptome. By now, only several traditional …

Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Data normalization is essential for reliable output of quantitative real-time reverse-
transcription polymerase chain reaction (qRT-PCR) assays, as the unsuitable choice of …

The real time quantitative reverse transcription PCR (qRT-PCR) is becoming increasingly
important to gain insight into function of genes. Given the increased sensitivity, ease and …

Quantitative real‐time reverse transcriptase polymerase chain reaction (qRT‐PCR) is an
effective method for quantifying expression levels of target genes. The accuracy of qRT …

Switchgrass (Panicum virgatum) has received a lot of attention as a forage and bioenergy
crop during the past few years. Gene expression studies are in progress to improve new …

Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and
accurate quantification of gene expression studies. Appropriate application of RT-qPCR …

Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …