Computational tool-assisted primer design for real-time reverse transcription (RT) PCR
(qPCR) analysis largely ignores the sequence similarities between sequences of …

The selection of reliable reference genes (RGs) for normalization under given experimental
conditions is necessary to develop an accurate qRT-PCR assay. To the best of our …

Stable and uniform expression of reference genes across samples plays a key role in
accurate normalization of gene expression by reverse-transcription quantitative polymerase …

Real‐time quantitative polymerase chain reaction (RT‐qPCR) has been widely applied in
gene expression and transcription abundance analysis because of its high sensitivity, good …

Quantitative real-time polymerase chain reaction (qRT-PCR) has been extensively used in
several plant species as an accurate technique for gene expression analysis. However, the …

Real-time reverse transcription quantitative PCR has become a common method for
studying gene expression, however, the optimal selection of stable reference genes is a …

Mulberry (Morus alba L.) is the sole food source for the mulberry silkworm, Bombyx mori and
therefore important for sericulture industry. Different abiotic stress conditions like drought …

The success of quantitative real-time reverse transcription polymerase chain reaction (RT-
qPCR) to quantify gene expression depends on the stability of the reference genes used for …

Miscanthus lutarioriparia, which is found widespread in China, has attracted great attention
as a most potential bioenergy plant for years. The quantitative real time PCR (RT-qPCR) has …

Quantitative real-time reverse transcription polymerase chain reaction (qPCR), a sensitive
technique for gene expression analysis, depends on the stability of the reference genes …