Moringa oleifera is a promising plant species for oil and forage, but its genetic improvement
is limited. Our current breeding program in this species focuses on exploiting the functional …

The quantitative real-time polymerase chain reaction (qRT-PCR) approach has become a
widely used method to analyze expression patterns of target genes. The selection of an …

Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique
capable of accurately characterizing gene expression. However, the lack of suitable …

Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular
technique for investigating gene expression levels in plants. Based on the Minimum …

Neolamarckia cadamba is an economically-important fast-growing tree species in South
China and Southeast Asia. As a prerequisite first step for future gene expression studies, we …

Background Reference genes are necessary for quantitative real-time PCR (qRT-PCR)
analysis and their stability can directly influence the accuracy of gene expression result …

Quantifying gene expression levels is an important research tool to understand biological
systems. Reverse transcription–quantitative real-time PCR (RT–qPCR) is the preferred …

Abstract qRT-PCR is becoming a routine tool in molecular biology to study gene expression.
It is necessary to find stable reference genes when performing qRT-PCR. The expression of …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Real time quantitative reverse transcription PCR (RT-qPCR) has been attracting more
attention for its high sensitivity in gene expression analysis. Given the widely use of RT …