Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis …

J Qi, S Yu, F Zhang, X Shen, X Zhao, Y Yu… - Plant Molecular Biology …, 2010 - Springer
The Chinese cabbage is a crop belonging to the family Cruciferae; very few studies have
focused on the analysis of gene expression in this economically important crop. In this study …

Identification of reference genes for transcript normalization in various tissue types and seedlings subjected to different abiotic stresses of woodland strawberry …

D Liu, X Huang, Y Lin, X Wang, Z Yan, Q Wang… - Scientia …, 2020 - Elsevier
Quantitative real-time PCR (qRT-PCR) is one of the most popular methods for gene
expression studies. However, data on the usage of reference genes in strawberry are …

[HTML][HTML] Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla

Y He, H Yan, W Hua, Y Huang, Z Wang - Frontiers in plant science, 2016 - frontiersin.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the …

Identification of suitable reference genes for normalization of real-time quantitative PCR data in pecan (Carya illinoinensis)

Z Mo, Y Chen, W Lou, X Jia, M Zhai, J Xuan, Z Guo… - Trees, 2020 - Springer
Key message This study proposed the combination of PR26S and PP1 as a good choice for
RT-qPCR normalization in pecan under abiotic stress, developing kernels, grafting, and …

[HTML][HTML] Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis

PK Martins, V Mafra, WR de Souza, AP Ribeiro… - Scientific reports, 2016 - nature.com
Abstract Real-time PCR (RT-qPCR) expression analysis is a powerful analytical technique,
but reliable results depend on the use of stable reference genes for proper normalization …

Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR

X Chi, R Hu, Q Yang, X Zhang, L Pan, N Chen… - Molecular Genetics and …, 2012 - Springer
Quantitative real-time reverse transcription PCR (qRT-PCR), a sensitive technique for
quantifying gene expression, depends on the stability of the reference gene (s) used for data …

Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions

HY Zhong, JW Chen, CQ Li, L Chen, JY Wu, JY Chen… - Plant cell reports, 2011 - Springer
Reverse transcription quantitative real-time PCR (RT-qPCR), a sensitive technique for
quantifying gene expression, depends on the stability of the reference gene (s) used for data …

[HTML][HTML] Expression Stabilities of Candidate Reference Genes for RT-qPCR in Chinese Jujube (Ziziphus jujuba Mill.) under a Variety of Conditions

J Bu, J Zhao, M Liu - PloS one, 2016 - journals.plos.org
Reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) is a
powerful method for evaluating patterns of gene expression. Jujube whole-genome …

Reference gene validation for qRT-PCR based gene expression studies in different developmental stages and under biotic stress in apple

G Kumar, AK Singh - Scientia Horticulturae, 2015 - Elsevier
Apple is one of the most important fruit crops and the availability of recently sequenced
genome facilitates research on gene–trait relationship. Quantitative real-time PCR (qRT …

[HTML][HTML] Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

H Sun, X Jiang, M Sun, H Cong, F Qiao - Plant Methods, 2019 - Springer
Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used
approach for investigating gene expression levels in plants because of its high …