Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

H Sun, X Jiang, M Sun, H Cong, F Qiao - Plant Methods, 2019 - Springer
Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used
approach for investigating gene expression levels in plants because of its high …

Reference Gene Selection for Gene Expression Studies Using Quantitative Real-Time PCR Normalization in Atropa belladonna

J Li, M Chen, F Qiu, B Qin, W Liu, N Wu, X Lan… - Plant molecular biology …, 2014 - Springer
Quantitative PCR (qPCR) is a powerful tool for measuring gene expression levels. Accurate
and reproducible results are dependent on the correct choice of reference genes for data …

Reference gene validation for qRT-PCR based gene expression studies in different developmental stages and under biotic stress in apple

G Kumar, AK Singh - Scientia Horticulturae, 2015 - Elsevier
Apple is one of the most important fruit crops and the availability of recently sequenced
genome facilitates research on gene–trait relationship. Quantitative real-time PCR (qRT …

Identification of suitable reference genes for normalization of RT-qPCR data in eggplant fruit and shoot borer (Leucinodes orbonalis Guenée)

B Kariyanna, A Prabhuraj, R Asokan, P Babu, SK Jalali… - Biologia, 2020 - Springer
The eggplant shoot and fruit borer, Leucinodes orbonalis Guenée (Lepidoptera: Crambidae)
is a monophagous and destructive insect pest of eggplant. L. orbonalis developed multiple …

Stable Internal Reference Genes for Normalizing Real-Time Quantitative PCR in Baphicacanthus cusia under Hormonal Stimuli and UV Irradiation, and in Different …

Y Huang, H Tan, J Yu, Y Chen, Z Guo… - Frontiers in plant …, 2017 - frontiersin.org
Baphicacanthus cusia (Nees) Bremek, the plant source for many kinds of drugs in traditional
Chinese medicine, is widely distributed in South China, especially in Fujian. Recent studies …

Identification of Reference Genes for qRT‐PCR Gene Expression Studies during Seed Development and under Abiotic Stresses in Cyamopsis tetragonoloba

PS Jaiswal, N Kaur, GS Randhawa - Crop Science, 2019 - Wiley Online Library
Guar [Cyamopsis tetragonoloba (L.) Taubert] is an important industrial crop. The knowledge
about genes of guar involved in various processes can help in developing improved …

Selection of Reliable Reference Genes for Gene Expression Studies of a Promising Oilseed Crop, Plukenetia volubilis, by Real-Time Quantitative PCR

L Niu, YB Tao, MS Chen, Q Fu, C Li, Y Dong… - International journal of …, 2015 - mdpi.com
Real-time quantitative PCR (RT-qPCR) is a reliable and widely used method for gene
expression analysis. The accuracy of the determination of a target gene expression level by …

Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments

X Niu, J Qi, G Zhang, J Xu, A Tao, P Fang… - Frontiers in plant …, 2015 - frontiersin.org
To accurately measure gene expression using quantitative reverse transcription PCR (qRT-
PCR), reliable reference gene (s) are required for data normalization. Corchorus capsularis …

Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis of Metasequoia

JJ Wang, S Han, W Yin, X Xia, C Liu - International Journal of Molecular …, 2018 - mdpi.com
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most
sensitive technique for evaluating gene expression levels. Choosing appropriate reference …

Validation of reference genes for quantitative RT-PCR normalization in Suaeda aralocaspica, an annual halophyte with heteromorphism and C4 pathway without …

J Cao, L Wang, H Lan - PeerJ, 2016 - peerj.com
Reverse transcription quantitative real-time polymerase chain reaction (qRT-PCR) is a
powerful analytical technique for the measurement of gene expression, which depends on …