Background Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput
technique to measure mRNA transcript levels. The accuracy of this evaluation of gene …

Selection and validation of appropriate reference genes should be the first step to consider
in experiments based on quantitative real-time polymerase chain reaction (qRT-PCR). In this …

Key message Evaluation and selection of reference genes in Pinus massoniana L.(PM) for
gene expression studies of various tissues, floral organ development, and abiotic stress …

Quantitative reverse transcription PCR (qRT-PCR) is one of the most precise and widely
used methods of gene expression analysis. A necessary prerequisite of exact and reliable …

Leaf pathogens are limiting factors in banana (Musa spp.) production, with
Pseudocercospora spp. responsible for the important Sigatoka disease complex. In order to …

Background The Moso bamboo (Phyllostachys edulis) is one of the most important forestry
resources and plays essential ecological roles in southern China. A draft nuclear genome …

Gene expression analysis is increasingly important in biological research, with reverse
transcription–quantitative PCR (RT–qPCR) becoming the method of choice for high …

Mei (Prunus mume) is widely cultivated in eastern Asia owing to its favored ornamental
characteristics and its tolerance for low temperatures. Reverse transcription quantitative real …

The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a powerful
and widely used technique for the measurement of gene expression. Reference genes …

Background Gene expression studies are a prerequisite for understanding the biological
function of genes. Because of its high sensitivity and easy use, quantitative PCR (qPCR) has …