Species are the fundamental units of life and evolution. Their recognition is essential for
science and society. Molecular methods have been increasingly used for the identification of …

Delineating species is a difficult and seemingly uninteresting issue that is still essential to
address. Taxonomic methodology is heterogeneous according to the taxa and scientists …

Delimiting species boundaries is central to understand ecological and evolutionary
processes, and to monitor biodiversity patterns over time and space. Yet, most of our current …

Singletons—species only known from a single specimen—and uniques—species that have
only been collected once—are very common in biodiversity samples. Recent reviews …

Delimitation of species based exclusively on genetic data has been advocated despite a
critical knowledge gap: how might such approaches fail because they rely on genetic data …

DNA barcoding methods use a single locus (usually the mitochondrial COI gene) to assign
unidentified specimens to known species in a library based on a genetic distance threshold …

The advent in high-throughput-sequencing (HTS) technologies has revolutionized
conventional biodiversity research by enabling parallel capture of DNA sequences …

MALTE C. EBACH AND CRAIG HOLDREGE ecologist and environmental consultant more
easily than we can imagine its value to taxonomy. Barcoding could indeed be valuable in …

Species are commonly delimited on the basis of gaps in patterns of morphological variation,
but there seems to be little recent work on methods to objectively assess such gaps. Here …

Taxonomy underpins all biological research, with implications for many basic scientific and
applied fields. Insights into the stability or change of animal and plant guilds require species …