Systematic selection and validation of appropriate reference genes for gene expression studies by quantitative real-time PCR in pear
Y Xu, H Li, X Li, J Lin, Z Wang, Q Yang… - Acta physiologiae …, 2015 - Springer
RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …
Identification of suitable reference genes for normalization of real-time quantitative PCR data in pecan (Carya illinoinensis)
Z Mo, Y Chen, W Lou, X Jia, M Zhai, J Xuan, Z Guo… - Trees, 2020 - Springer
Key message This study proposed the combination of PR26S and PP1 as a good choice for
RT-qPCR normalization in pecan under abiotic stress, developing kernels, grafting, and …
RT-qPCR normalization in pecan under abiotic stress, developing kernels, grafting, and …
[HTML][HTML] Selection of appropriate reference genes for RT-qPCR analysis under abiotic stress and hormone treatment in celery
K Feng, J Liu, GM Xing, S Sun, S Li, AQ Duan, F Wang… - PeerJ, 2019 - peerj.com
Celery is one of the most important vegetable crop and its yield and quality is influenced by
many environmental factors. Researches on gene expression not only help to unravel the …
many environmental factors. Researches on gene expression not only help to unravel the …
Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions
HY Zhong, JW Chen, CQ Li, L Chen, JY Wu, JY Chen… - Plant cell reports, 2011 - Springer
Reverse transcription quantitative real-time PCR (RT-qPCR), a sensitive technique for
quantifying gene expression, depends on the stability of the reference gene (s) used for data …
quantifying gene expression, depends on the stability of the reference gene (s) used for data …
[HTML][HTML] Selection and identification of a reference gene for normalizing real-time PCR in mangos under various stimuli in different tissues
R Yao, X Huang, H Cong, F Qiao, Y Cheng, Y Chen - Horticulturae, 2022 - mdpi.com
Real-time quantitative polymerase chain reaction (Real-Time PCR) is a rapid, highly
sensitive, and highly specific technique, which is widely used to determine the relative …
sensitive, and highly specific technique, which is widely used to determine the relative …
[HTML][HTML] Evaluation of Reference Genes for Accurate Normalization of Gene Expression for Real Time-Quantitative PCR in Pyrus pyrifolia Using Different Tissue …
T Imai, BE Ubi, T Saito, T Moriguchi - PLoS One, 2014 - journals.plos.org
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR)
analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the …
analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the …
[HTML][HTML] Selection of reliable reference genes for gene expression studies in peach using real-time PCR
Z Tong, Z Gao, F Wang, J Zhou, Z Zhang - BMC molecular biology, 2009 - Springer
Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …
[PDF][PDF] Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress.
ZL Duan, WH Han, L Yan, B Wu - Biologia plantarum, 2020 - bp.ueb.cas.cz
Appropriate choice of reference genes for data normalization is of critical importance for
accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene …
accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene …
[HTML][HTML] Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions
X Zhu, X Li, W Chen, J Chen, W Lu, L Chen, D Fu - 2012 - journals.plos.org
Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and
accurate quantification of gene expression studies. Appropriate application of RT-qPCR …
accurate quantification of gene expression studies. Appropriate application of RT-qPCR …
[HTML][HTML] Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development
X Han, M Lu, Y Chen, Z Zhan, Q Cui, Y Wang - 2012 - journals.plos.org
Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …
to analyze expression levels of selected genes. It is very necessary to select appropriate …