Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR
M Hu, W Hu, Z Xia, X Zhou, W Wang - Frontiers in Plant Science, 2016 - frontiersin.org
Reverse transcription quantitative real-time polymerase chain reaction (real-time PCR, also
referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput …
referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput …
Identification and Evaluation of Reliable Reference Genes for Quantitative Real-Time PCR Analysis in Tea Plant (Camellia sinensis (L.) O. Kuntze)
X Hao, DP Horvath, WS Chao, Y Yang, X Wang… - International journal of …, 2014 - mdpi.com
Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …
Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla
Y He, H Yan, W Hua, Y Huang, Z Wang - Frontiers in plant science, 2016 - frontiersin.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the …
gene expression because of its accuracy, sensitivity, and high throughput. However, the …
Comparative expression analysis of reference genes in field-grown cassava
A Salcedo, C Zambrana, D Siritunga - Tropical plant biology, 2014 - Springer
Due to its accuracy, sensitivity and reproducibility, real-time PCR has emerged as a key
technique to measure changes in expression of target genes. But to obtain reliable results …
technique to measure changes in expression of target genes. But to obtain reliable results …
Selection of reference genes for quantitative real-time PCR in six oil-tea camellia based on RNA-seq
CF Zhou, P Lin, XH Yao, KL Wang, J Chang, XJ Han - Molecular Biology, 2013 - Springer
Abstract qRT-PCR is becoming a routine tool in molecular biology to study gene expression.
It is necessary to find stable reference genes when performing qRT-PCR. The expression of …
It is necessary to find stable reference genes when performing qRT-PCR. The expression of …
Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber–pumpkin …
L Miao, X Qin, L Gao, Q Li, S Li, C He, Y Li, X Yu - PeerJ, 2019 - peerj.com
Background Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput
technique to measure mRNA transcript levels. The accuracy of this evaluation of gene …
technique to measure mRNA transcript levels. The accuracy of this evaluation of gene …
Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions
L Chen, H Zhong, J Kuang, J Li, W Lu, J Chen - Planta, 2011 - Springer
Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique for
quantifying gene expression, but its success depends on the stability of the reference gene …
quantifying gene expression, but its success depends on the stability of the reference gene …
Selection of suitable reference genes for reverse transcription quantitative real-time PCR studies on different experimental systems from carrot (Daucus carota L.)
MD Campos, AM Frederico, T Nothnagel… - Scientia …, 2015 - Elsevier
Reverse transcription quantitative real time polymerase chain reaction (RT-qPCR) is a
preferred method for rapid and accurate quantification of gene expression studies …
preferred method for rapid and accurate quantification of gene expression studies …
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR
H Ling, Q Wu, J Guo, L Xu, Y Que - PloS one, 2014 - journals.plos.org
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method
for gene expression analysis requires one or several reference gene (s) acting as …
for gene expression analysis requires one or several reference gene (s) acting as …
Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development
X Han, M Lu, Y Chen, Z Zhan, Q Cui, Y Wang - 2012 - journals.plos.org
Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …
to analyze expression levels of selected genes. It is very necessary to select appropriate …