Real-time quantitative RT-PCR (qRT-PCR) is one of the most common technologies used for
gene expression and transcriptome analysis, with its high sensitivity, specificity, good …

Quantitative RT-PCR (qRT-PCR) has been widely used in gene expression analysis
because of its sensitivity, specificity, and reproducibility. Application of suitable reference …

Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Accurate quantitative reverse transcription PCR (qRT-PCR) requires reliable reference
genes whose expression does not vary in different tissues and developmental stages …

With a widely established use of quantitative real-time PCR (qRT-PCR) for gene expression
analysis, reliable and stable expression of reference genes is often discussed. Suitable …

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is extensively
applied technique to investigate the transcript abundance of target genes in various …

Quantifying gene expression levels is an important research tool to understand biological
systems. Reverse transcription–quantitative real-time PCR (RT–qPCR) is the preferred …

Quantitative real-time reverse transcription PCR (qRT-PCR) analysis has been used
routinely to quantify gene expression levels. Primer design and the optimization of qRT-PCR …

Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the …

Computational tool-assisted primer design for real-time reverse transcription (RT) PCR
(qPCR) analysis largely ignores the sequence similarities between sequences of …