We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR)
analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the …

Quantitative real-time PCR (RT-qPCR) has become an accurate and widely used technique
to analyze expression levels of selected genes. It is very necessary to select appropriate …

Key message This study proposed the combination of PR26S and PP1 as a good choice for
RT-qPCR normalization in pecan under abiotic stress, developing kernels, grafting, and …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Reliable reference selection for the accurate quantification of gene expression under
various experimental conditions is a crucial step in qRT-PCR normalization. To date, only a …

Quantitative real-time polymerase chain reaction (qRT-PCR) has been extensively used in
several plant species as an accurate technique for gene expression analysis. However, the …

RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …

Quantitative RT-PCR (qRT-PCR) has been widely used in gene expression analysis
because of its sensitivity, specificity, and reproducibility. Application of suitable reference …

Background A suitable reference gene is an important prerequisite for guarantying accurate
and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no …

In this study, eight candidate reference genes including EF1α, TUB-b2, GAPDH, 18S rRNA,
RP-L27P, UBI, ACT and ACT2 were selected to evaluate expression stability in pear fruit of …