Developmental differentiation is a universal biological process that allows cells to adapt to
different environments to perform specific functions. African trypanosomes progress through …

Trypanosoma cruzi is the etiological agent for Chagas disease, a neglected parasitic
disease in Latin America. Gene transcription control governs the eukaryotic cell replication …

A major obstacle in understanding the complex biology of the malaria parasite remains to
discover how gene transcription is controlled during its life cycle. Accumulating evidence …

Recent studies have identified histone modifications and suggested a role for epigenetic
gene regulation in Trypanosoma brucei. The histone modification H4K10ac and histone …

Replication of Trypanosoma cruzi, the etiological agent of Chagas disease, displays
peculiar features, such as absence of chromosome condensation and closed mitosis …

Systems analysis of chromatin has been constrained by complex patterns and dynamics of
histone post-translational modifications (PTMs), which represent major challenges for both …

The structure, genomic organization and transcription of the gene encoding histone H2B in
the protozoan parasite Trypanosoma cruzi have been studied. This gene consists of a 746 …

Nucleosomes composed of histones are the fundamental units around which DNA is
wrapped to form chromatin. Transcriptionally active euchromatin or repressive …

In yeast and mammalian cells, the cell cycle-dependent histone genes are typically
expressed at a 15-to 35-fold higher level during S phase than during other phases of the cell …

We describe a single-step centrifugal elutriation method to produce synchronous Gap1 (G1)-
phase procyclic trypanosomes at a scale amenable for proteomic analysis of the cell cycle …