Different amplicon targets for sequencing-based studies of fungal diversity

F De Filippis, M Laiola, G Blaiotta… - Applied and …, 2017 - Am Soc Microbiol
Target-gene amplicon sequencing is the most exploited high-throughput sequencing
application in microbial ecology. The targets are taxonomically relevant genes, with 16S …

Factors that affect large subunit ribosomal DNA amplicon sequencing studies of fungal communities: classification method, primer choice, and error

TM Porter, GB Golding - PLoS One, 2012 - journals.plos.org
Nuclear large subunit ribosomal DNA is widely used in fungal phylogenetics and to an
increasing extent also amplicon-based environmental sequencing. The relatively short …

New primers to amplify the fungal ITS2 region–evaluation by 454-sequencing of artificial and natural communities

K Ihrmark, ITM Bödeker, K Cruz-Martinez… - FEMS microbiology …, 2012 - academic.oup.com
With recent methodological advances, molecular markers are increasingly used for semi-
quantitative analyses of fungal communities. The aim to preserve quantitative relationships …

Exploring the accuracy of amplicon‐based internal transcribed spacer markers for a fungal community

S Li, YE Deng, Z Wang, Z Zhang, X Kong… - Molecular Ecology …, 2020 - Wiley Online Library
With the continual improvement in high‐throughput sequencing technology and constant
updates to fungal reference databases, the use of amplicon‐based DNA markers as a tool to …

A new fungal large subunit ribosomal RNA primer for high-throughput sequencing surveys

RC Mueller, LV Gallegos-Graves… - FEMS Microbiology …, 2016 - academic.oup.com
The inclusion of phylogenetic metrics in community ecology has provided insights into
important ecological processes, particularly when combined with high-throughput …

The ITS region as a target for characterization of fungal communities using emerging sequencing technologies

RH Nilsson, M Ryberg, K Abarenkov… - FEMS Microbiology …, 2009 - academic.oup.com
The advent of new high-throughput DNA-sequencing technologies promises to redefine the
way in which fungi and fungal communities–as well as other groups of organisms–are …

Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities

AL Bazzicalupo, M Balint, I Schmitt - Fungal Ecology, 2013 - Elsevier
Next-generation amplicon sequencing is a powerful tool in ecological studies of fungi.
Technological development suggests that short fragment high-throughput techniques, eg …

Generating amplicon reads for microbial community assessment with next‐generation sequencing

M Gołębiewski, A Tretyn - Journal of Applied Microbiology, 2020 - academic.oup.com
Marker gene amplicon sequencing is often preferred over whole genome sequencing for
microbial community characterization, due to its lower cost while still enabling assessment of …

Don't make a mista (g) ke: is tag switching an overlooked source of error in amplicon pyrosequencing studies?

T Carlsen, AB Aas, D Lindner, T Vrålstad… - Fungal Ecology, 2012 - Elsevier
High throughput sequencing has become a powerful tool for fungal ecologists to explore the
diversity and composition of fungal communities. However, various biases and errors are …

Accurate estimation of fungal diversity and abundance through improved lineage-specific primers optimized for Illumina amplicon sequencing

DL Taylor, WA Walters, NJ Lennon… - Applied and …, 2016 - Am Soc Microbiol
While high-throughput sequencing methods are revolutionizing fungal ecology, recovering
accurate estimates of species richness and abundance has proven elusive. We sought to …