[HTML][HTML] Critical issues in mycobiota analysis

B Halwachs, N Madhusudhan, R Krause… - Frontiers in …, 2017 - frontiersin.org
Fungi constitute an important part of the human microbiota and they play a significant role for
health and disease development. Advancements made in the culture-independent analysis …

[HTML][HTML] CloVR-ITS: automated internal transcribed spacer amplicon sequence analysis pipeline for the characterization of fungal microbiota

JR White, C Maddox, O White, SV Angiuoli, WF Fricke - Microbiome, 2013 - Springer
Background Besides the development of comprehensive tools for high-throughput 16S
ribosomal RNA amplicon sequence analysis, there exists a growing need for protocols …

Fungal identification biases in microbiome projects

L Tedersoo, B Lindahl - Environmental microbiology reports, 2016 - Wiley Online Library
Fungi are the key players in ecosystems as well as in plant and human health. High‐
throughput molecular identification of fungi has greatly progressed our understanding about …

[HTML][HTML] HumanMycobiomeScan: a new bioinformatics tool for the characterization of the fungal fraction in metagenomic samples

M Soverini, S Turroni, E Biagi, P Brigidi, M Candela… - BMC genomics, 2019 - Springer
Background Modern metagenomic analysis of complex microbial communities produces
large amounts of sequence data containing information on the microbiome in terms of …

[HTML][HTML] MICCA: a complete and accurate software for taxonomic profiling of metagenomic data

D Albanese, P Fontana, C De Filippo, D Cavalieri… - Scientific reports, 2015 - nature.com
The introduction of high throughput sequencing technologies has triggered an increase of
the number of studies in which the microbiota of environmental and human samples is …

Novel ITS1 fungal primers for characterization of the mycobiome

M Usyk, CP Zolnik, H Patel, MH Levi, RD Burk - MSphere, 2017 - Am Soc Microbiol
Studies of the human microbiome frequently omit characterization of fungal communities
(the mycobiome), which limits our ability to investigate how fungal communities influence …

Mycobiome: approaches to analysis of intestinal fungi

J Tang, ID Iliev, J Brown, DM Underhill… - Journal of immunological …, 2015 - Elsevier
Massively parallel sequencing (MPSS) of bacterial 16S rDNA has been widely used to
characterize the microbial makeup of the human and mouse gastrointestinal tract. However …

[HTML][HTML] CONSTAX: a tool for improved taxonomic resolution of environmental fungal ITS sequences

K Gdanetz, GMN Benucci, N Vande Pol, G Bonito - BMC bioinformatics, 2017 - Springer
Background One of the most crucial steps in high-throughput sequence-based microbiome
studies is the taxonomic assignment of sequences belonging to operational taxonomic units …

[HTML][HTML] Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese …

D Motooka, K Fujimoto, R Tanaka, T Yaguchi… - Frontiers in …, 2017 - frontiersin.org
The study of mycobiota remains relatively unexplored due to the lack of sufficient available
reference strains and databases compared to those of bacterial microbiome studies. Deep …

Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …