Metagenomic shotgun sequencing (MSS) is an important tool for characterizing viral
populations. It is culture independent, requires no a priori knowledge of the viruses in the …

Insensitivity and technical complexity have impeded the implementation of high-throughput
nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories …

Recent advances in sequencing technologies have transformed the field of virus discovery
and virome analysis. Once mostly confined to the traditional Sanger sequencing based …

Objectives Sample preparation for high-throughput sequencing (HTS) includes treatment
with various laboratory components, potentially carrying viral nucleic acids, the extent of …

Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic
testing that allows simultaneous detection of a broad range of viruses, detailed taxonomic …

Highlights•New amplification methods reduce biases caused by whole genome
amplification•Biases caused by assembly can be avoided using long-read …

Accurate classification of the human virome is critical to a full understanding of the role
viruses play in health and disease. This implies the need for sensitive, specific, and practical …

Unbiased metagenomic sequencing holds significant potential as a diagnostic tool for the
simultaneous detection of any previously genetically described viral nucleic acids in clinical …

Metagenomics, ie, the sequencing and analysis of genomic information extracted directly
from clinical or environmental samples, has become a fundamental tool to explore the viral …

The target-independent identification of viral pathogens using 'shotgun'metagenomic
sequencing is an emerging approach with potentially wide applications in clinical …