Background Quantifying gene expression is a critical aspect of applied genomics research
across all organisms, and real-time PCR has emerged as a powerful tool for this purpose …

Parsley, one of the most important vegetables in the Apiaceae family, is widely used in the
food, medicinal, and cosmetic industries. Recent studies on parsley mainly focus on its …

Pearl millet [Pennisetum glaucum (L.) R. Br.] a widely used grain and forage crop, is grown
in areas frequented with one or more abiotic stresses, has superior drought and heat …

Lycoris aurea (L'Hér.) Herb, a perennial grass species, produces a unique variety of
pharmacologically active Amaryllidaceae alkaloids. However, the key enzymes and their …

Finger millet [Eleusine coracana (L.) Gaertn] is an excellent food and forage crop of arid and
semiarid areas in Africa and Asia. It is well adapted to drought, heat, high salinity, poor soil …

Eustoma grandiflorum, commonly known as prairie gentian or Texas bluebells, is among the
most popular agriculturally propagated species of cut flowers. Due to its widespread appeal …

To facilitate gene expression studies in pearl millet (Pennisetum glaucum (L.) R. Br.), the key
reference genes including ACP, ACT, TUB, CYP, EF-1α, EIF4A, GAPDH, MDH, PP2C, UBC …

Reverse transcription quantitative real-time polymerase chain reaction (PCR) is a widely
used and reproducible method for studying gene expression changes. However, its …

Lycium barbarum and L. ruthenicum are extensively used as traditional Chinese medicinal
plants. Next generation sequencing technology provides a powerful tool for analyzing …

The appropriate reference genes are crucial for normalization of the target gene expression
in qRT-PCR analysis. Broomcorn millet (Panicum miliaceum L.) is one of the most important …