The appropriate reference genes are crucial for normalization of the target gene expression
in qRT-PCR analysis. Broomcorn millet (Panicum miliaceum L.) is one of the most important …

Pearl millet [Pennisetum glaucum (L.) R. Br.] a widely used grain and forage crop, is grown
in areas frequented with one or more abiotic stresses, has superior drought and heat …

Quantitative real-time polymerase chain reaction (RT-qPCR) using a stable reference gene
is widely used for gene expression research. Barnyard millet (Echinochloa spp.) is an …

Quantitative real-time PCR (qRT-PCR) has been widely used for studying gene expression
at the transcript level. Its accuracy usually relies on the reference genes that are utilized for …

Selecting an appropriate reference gene is of crucial importance for improving the accuracy
of qRT-PCR analyses. In this study, strawberry (Fragaria ananassa) seedlings were …

Background Reference genes are necessary for quantitative real-time PCR (qRT-PCR)
analysis and their stability can directly influence the accuracy of gene expression result …

To facilitate gene expression studies in pearl millet (Pennisetum glaucum (L.) R. Br.), the key
reference genes including ACP, ACT, TUB, CYP, EF-1α, EIF4A, GAPDH, MDH, PP2C, UBC …

Background Because of its strong specificity and high accuracy, real-time quantitative PCR
(RT-qPCR) has been a widely used method to study the expression of genes responsive to …

Real-time quantitative polymerase chain reaction (Real-Time PCR) is a rapid, highly
sensitive, and highly specific technique, which is widely used to determine the relative …

Abstract Background Quantitative PCR (qPCR) is one of the most common and accurate
methods of gene expression analysis. However, the biggest challenge for this kind of …