Selecting appropriate reference gene is key step for the accurate analysis of the expression
variations of target genes. Using several common-used reference genes of SAND-1, ACT …

Reference genes are frequently used as a normalization standard to obtain reliable data
during quantitative real-time polymerase chain reaction (qRT-PCR). However, recent studies …

Lentil (Lens culinaris) is one of the most important staple food crops of developing countries.
Transcriptome based global gene expression profiling followed by validation of expression …

Grass pea (Lathyrus sativus L.) is a robust legume crop with high protein content and good
stress tolerance. However, it needs genetic improvement due to the presence of several anti …

Real-time quantitative PCR (RT-qPCR) has a high sensitivity and strong specificity, and is
widely used in the analysis of gene expression. Selecting appropriate internal reference …

Pearl millet [Pennisetum glaucum (L.) R. Br.], a close relative of Panicoideae food crops and
bioenergy grasses, offers an ideal system to perform functional genomics studies related to …

Gene expression analysis is increasingly important in biological research, with reverse
transcription–quantitative PCR (RT–qPCR) becoming the method of choice for high …

Gene expression studies could provide insight into the physiological mechanisms and
strategies used by plants under stress conditions. Selection of suitable internal control gene …

Quantitative PCR (qPCR) is a powerful tool for measuring gene expression levels. Accurate
and reproducible results are dependent on the correct choice of reference genes for data …

The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind
prevention, and desert ecosystem recovery. An absence of reference genes greatly limits …