Cryopreservation of bovinein VitroProduced embryos using ethylene glycol in controlled freezing or vitrification

V Sommerfeld, H Niemann - Cryobiology, 1999 - Elsevier
In this study, the cryoprotectant ethylene glycol (EG) was tested for its ability to improve and
facilitate the cryopreservation ofin vitroproduced (IVP) bovine embryos. Embryos were …

Use of ethylene glycol as a cryoprotectant for bovine embryos allowing direct transfer of frozen-thawed embryos to recipient females

SA Voelkel, YX Hu - Theriogenology, 1992 - Elsevier
Four experiments were conducted to define a system for the direct transfer of frozen-thawed
bovine embryos to recipient females. In Experiment I, nonsurgically recovered embryos were …

Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro-derived bovine blastocysts

S Ohboshi, N Fujihara, T Yoshida… - Animal reproduction …, 1997 - Elsevier
A series of five experiments measured the high survival of bovine blastocysts produced in
vitro after cryopreservation by vitrification. The vitrification solution (designated VS) …

Direct transfer of bovine embryos frozen-thawed in the presence of propylene glycol or ethylene glycol under on-farm conditions in an integrated embryo transfer …

O Dochi, Y Yamamoto, H Saga, N Yoshiba, N Kano… - Theriogenology, 1998 - Elsevier
An integrated bovine embryo transfer program was conducted in collaboration with 11
Japanese prefectural livestock experiment stations. The program was conducted to evaluate …

Direct transfer of frozen-thawed bovine embryos

SA Voelkel, YX Hu - Theriogenology, 1992 - Elsevier
The ability to transfer frozen embryos directly to recipient females after thawing would be a
convenience in embryo transfer and would facilitate marketing embryos produced in large …

Birth of calves after direct transfer of thawed bovine embryos stored frozen in ethylene glycol

O Dochi, K Imai, H Takakura - Animal Reproduction Science, 1995 - Elsevier
The present study investigated the use of ethylene glycol (EG) as a cryoprotectant before the
direct transfer of frozen-thawed bovine embryos. Embryos at the morula to blastocyst stages …

Pregnancy rates after transfer of frozen bovine embryos: a field trial

AG Martınez, GM Brogliatti, A Valcarcel… - Theriogenology, 2002 - Elsevier
An efficient and practical technique for bovine embryo cryopreservation is a fundamental
issue in the widespread use of embryo transfer. The present study shows results obtained in …

Survival of IVF-derived bovine embryos frozen in glycerol or ethylene glycol

JF Hasler, PJ Hurtgen, ZQ Jin, JE Stokes - Theriogenology, 1997 - Elsevier
Survival of IVF-derived bovine embryos of different ages and stages of development,
produced in 2 different co-culture systems and frozen in 2 different cryoprotectants, was …

Freezing of bovine embryos: effects of a one-step addition of 1.4 M glycerol

H Niemann - Theriogenology, 1985 - Elsevier
The effects of a one-step addition of 1.4 M glycerol (method A) upon morphological
appearance and developmental capacity of frozen/thawed day 7 bovine embryos were …

Cryopreservation of ovine embryos: slow freezing and vitrification

AG Martinez, M Matkovic - Theriogenology, 1998 - Elsevier
Different methods for the cryopreservation of ovine embryos were evaluated in vitro (survival
upon culture in vitro) and in vivo (pregnancy and lambing rates after transfer in field …