Experiments were designed to determine optimal conditions for the cryopreservation of
bovine embryos produced in vitro. In Expt 1, embryos were exposed for 1, 3 or 5 min to a …

The effect of cooling and warming rates during cryopreservation on subsequent embryo
survival was studied in 607 bovine morulae and 595 blastocysts produced by in vitro …

In 5 replicates a total of 719 immature oocytes recovered from 94 slaughterhouse-derived
bovine ovaries were matured and fertilized in vitro, then cultured for 7 to 9 d on a granulosa …

Day 7 bovine morulae and early blastocysts were randomly assigned to one of four
cryopreservation methods:(i) a modified conventional controlled slow freezing and stepwise …

Cryopreservation describes techniques that permit freezing and subsequent warming of
biological samples without loss of viability. The application of cryopreservation in assisted …

Embryo cryopreservation represents a pivotal tool for the long-term storage and exchange of
valuable genetic resources of livestock and endangered species. The innumerable …

The in vitro survival of control and rapidly cryopreserved sheep embryos was examined as a
function of the duration of exposure to a vitrification medium (25% glycerol+ 25% propylene …

This study evaluated the efficiency and toxicity of two cryopreservation methods, solid-
surface vitrification (SSV) and cryoloop vitrification (CLV), on in vitro matured oocytes and in …

Two studies were conducted to evaluate the influence of cryoprotectant, cooling rate,
container and cryopreservation procedure on the post-thaw viability of sheep embryos. In …

The preservation of the female portion of livestock genetics has become an international
priority; however, in situ conservation strategies are extremely expensive. Therefore, efforts …