Animal oocyte and embryo cryopreservation

RM Pereira, CC Marques - Cell and tissue banking, 2008 - Springer
Cryopreservation of oocytes and embryos is a crucial step for the widespread and
conservation of animal genetic resources. However, oocytes and early embryos are very …

Cryopreservation of ovine embryos: slow freezing and vitrification

AG Martinez, M Matkovic - Theriogenology, 1998 - Elsevier
Different methods for the cryopreservation of ovine embryos were evaluated in vitro (survival
upon culture in vitro) and in vivo (pregnancy and lambing rates after transfer in field …

A comparison of cryotop and solid surface vitrification methods for the cryopreservation of in vitro matured bovine oocytes

N Sripunya, T Somfai, Y Inaba, T Nagai… - … of Reproduction and …, 2010 - jstage.jst.go.jp
The aim of the present study was to compare the efficacies of the cooling systems of the
solid surface (SSV) and Cryotop vitrification methods for cryopreservation of bovine oocytes …

The Effect of Long‐term In Vivo Culture in Bovine Oviduct and Uterus on the Development and Cryo‐tolerance of In Vitro Produced Bovine Embryos

V Havlicek, A Kuzmany, S Cseh, G Brem… - … in domestic animals, 2010 - Wiley Online Library
Contents The objective of this study was to compare the embryo production and quality
carried out entirely in vitro or partly in vitro combined with short‐vs long‐term in vivo culture …

Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium

C Cuello, CA Martinez, A Nohalez, I Parrilla, J Roca… - Scientific Reports, 2016 - nature.com
The use of pH-stable media would simplify embryo vitrification and the warming of porcine
embryos and might facilitate the application of embryo transfer in practice. In this work, we …

Effect of container, vitrification volume and warming solution on cryosurvival of in vitro-produced bovine embryos

GL Rios, NC Mucci, GG Kaiser, RH Alberio - Animal reproduction science, 2010 - Elsevier
The aim of the present research was to develop a low cost and easy to perform vitrification
method for in vitro-produced cattle embryos. Effect of container material was evaluated …

Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes

Y Liu, Y Du, L Lin, J Li, PM Kragh, M Kuwayama… - …, 2008 - ingentaconnect.com
During the past few years vitrification has been acknowledged as a viable alternative to
traditional slow-rate freezing in both animal and human embryology. However, few data are …

Inhibition of apoptosis by caspase inhibitor Z-VAD-FMK improves cryotolerance of in vitro derived bovine embryos

ME Pero, G Zullo, L Esposito, A Iannuzzi, P Lombardi… - Theriogenology, 2018 - Elsevier
The aim of this work was to evaluate whether the treatment with the pan-caspase inhibitor
benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) during cryopreservation …

Generation of Live Piglets from Cryopreserved Oocytes for the First Time Using a Defined System for In Vitro Embryo Production

T Somfai, K Yoshioka, F Tanihara, H Kaneko… - PLoS …, 2014 - journals.plos.org
We report the successful piglet production from cryopreserved oocytes for the first time by
using a simple, high capacity vitrification protocol for preservation and a defined system for …

Cryopreservation of preimplantation embryos of cattle, sheep, and goats

CR Youngs - JoVE (Journal of Visualized Experiments), 2011 - jove.com
Preimplantation embryos from cattle, sheep, and goats may be cryopreserved for short-or
long-term storage. Preimplantation embryos consist predominantly of water, and the …