Establishment of Transcriptional Gene Silencing Targeting the Promoter Regions of GFP, PDS, and PSY Genes in Cotton using Virus-Induced Gene Silencing

AH Khan, A Akram, M Saeed, M ur Rahman… - Molecular …, 2023 - Springer
Virus-induced gene silencing (VIGS) by deploying viral-based vectors such as tobacco rattle
virus (TRV) is a homology-based gene silencing technique in post-transcriptional gene …

Applications of Virus-Induced Gene Silencing in Cotton

Y Tian, Y Fang, K Zhang, Z Zhai, Y Yang, M He, X Cao - Plants, 2024 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that
has become an effective tool to investigate gene function in plants. Cotton is one of the most …

Visible gland constantly traces virus-induced gene silencing in cotton

Z Si, H Wu, Y Tian, Z Zhang, T Zhang… - Frontiers in Plant Science, 2022 - frontiersin.org
A virus-induced gene silencing (VIGS) system was established to induce endogenous target
gene silencing by post-transcriptional gene silencing (PTGS), which is a powerful tool for …

Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing

X Gao, L Shan - Virus-Induced Gene Silencing: Methods and Protocols, 2013 - Springer
Cotton (Gossypium spp.) is one of the most agronomically important crops worldwide for its
unique textile fiber production and serving as food and feed stock. Molecular breeding and …

Tobacco Rattle Virus-Induced Gene Silencing in Cotton

C Cai, X Wang, B Zhang, W Guo - Transgenic Cotton: Methods and …, 2019 - Springer
Virus-induced gene silencing (VIGS), as a tool for plant reverse genetics, has been widely
used in cotton for target gene function analysis. Compared with genetically transformed …

Virus-Induced Gene Silencing in Cultivated Cotton (Gossypium spp.) Using Tobacco Rattle Virus

R Mustafa, M Shafiq, S Mansoor, RW Briddon… - Molecular …, 2016 - Springer
The study described here has optimized the conditions for virus-induced gene silencing
(VIGS) in three cultivated cotton species (Gossypium hirsutum, G. arboreum, and G …

Epigenetic silencing in transgenic plants

S Rajeevkumar, P Anunanthini… - Frontiers in plant …, 2015 - frontiersin.org
Epigenetic silencing is a natural phenomenon in which the expression of genes is regulated
through modifications of DNA, RNA, or histone proteins. It is a mechanism for defending host …

Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using Rice tungro bacilliform virus (RTBV) as a Vector

R Kant, S Sharma, I Dasgupta - Plant Gene Silencing: Methods and …, 2015 - Springer
The large-scale functional analysis of genes in plants depends heavily on robust techniques
for gene silencing. Virus-induced gene silencing (VIGS) is a transient gene silencing method …

Virus-induced gene silencing (VIGS): a powerful tool for crop improvement and its advancement towards epigenetics

S Zulfiqar, MA Farooq, T Zhao, PP Wang… - International journal of …, 2023 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that
has evolved into an indispensable approach for analyzing the function of genes. It …

Induction of RNA-directed DNA methylation and heritable transcriptional gene silencing as a tool to engineer novel traits in plants

M Kasai, A Kanazawa - Plant Biotechnology, 2013 - jstage.jst.go.jp
Gene silencing through transcriptional repression can be induced by double-stranded RNA
(dsRNA) that targets a gene promoter. This phenomenon, termed RNA-mediated …