A Facile Platform to Engineer Escherichia coli Tyrosyl-tRNA Synthetase Adds New Chemistries to the Eukaryotic Genetic Code, Including a Phosphotyrosine Mimic
KT Grasso, SJ Singha Roy, AO Osgood… - ACS central …, 2022 - ACS Publications
The Escherichia coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNAEcTyr pair offers an attractive
platform for genetically encoding new noncanonical amino acids (ncAA) in eukaryotes.
However, challenges associated with a eukaryotic selection system, which is needed to
engineer the platform, have impeded its success in the past. Recently, using a facile E. coli-
based selection system, we showed that EcTyrRS could be engineered in a strain where the
endogenous tyrosyl pair was substituted with an archaeal counterpart. However, significant …
platform for genetically encoding new noncanonical amino acids (ncAA) in eukaryotes.
However, challenges associated with a eukaryotic selection system, which is needed to
engineer the platform, have impeded its success in the past. Recently, using a facile E. coli-
based selection system, we showed that EcTyrRS could be engineered in a strain where the
endogenous tyrosyl pair was substituted with an archaeal counterpart. However, significant …
A facile platform to engineer E. coli tyrosyl-tRNA synthetase adds new chemistries to the eukaryotic genetic code, including a phosphotyrosine mimic
The E. coli tyrosyl-tRNA synthetase (EcTyrRS)/tRNAEcTyr pair offers an attractive platform to
genetically encode new noncanonical amino acids (ncAA) in eukaryotes. However,
challenges associated with a eukaryotic selection system, which is needed for its
engineering, has impeded its success in the past. Recently, we showed that EcTyrRS can be
engineered using a facile E. coli based selection system, in a strain where the endogenous
tyrosyl pair has been substituted with an archaeal counterpart. However, a significant cross …
genetically encode new noncanonical amino acids (ncAA) in eukaryotes. However,
challenges associated with a eukaryotic selection system, which is needed for its
engineering, has impeded its success in the past. Recently, we showed that EcTyrRS can be
engineered using a facile E. coli based selection system, in a strain where the endogenous
tyrosyl pair has been substituted with an archaeal counterpart. However, a significant cross …
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