… simple, and highly efficient genetic manipulation techniques, the broader … Filamentous fungi,
such as Trichoderma reesei, Aspergillus niger, Aspergillus oryzae, and Aspergillus nidulans…

… and the sensitivity of the detection technique used. We previously showed that the well-characterized
soil filamentous fungus Aspergillus nidulans was highly affected by a commercial …

… Filamentous fungal hosts are often preferred for the expression of BGCs from filamentous
fungi… secretion systems, and overall better genetic compatibility than bacterial or yeast hosts [3, …

… TOOL 4: SELECTION MARKER SHUTTLE VECTOR In order to create an efficient shuttle
vector for E. coli and filamentous fungi we made use of the pki promoter of Trichoderma reesei. …

… Highly polarized growth of filamentous fungi requires a … to visualize actin cables in filamentous
fungi, the exact … fluorescent proteins in living Aspergillus nidulans hyphae and studied the …

… oryzae, Rhopzus oryzae, and Aspergillus nidulans. … safer gene editing in the future using
the CRISPR/Cas9 system. This effective method has not been used in the filamentous fungi, …

… Filamentous fungi are highly productive cell factories, often used … species, and as we use
established genetic elements … Manipulations in the Filamentous Fungus Aspergillus Nidulans. …

… mutation rate of filamentous fungal species and find that Aspergillus genomes are … in
Aspergillus nidulans was estimated to be 2.26 × 10 −10 using the classical Bateman-Mukai method […

… The work described here provides a new method for rapidly constructing gene disruption
vectors to create gene mutations with high efficiency compared with existing methods. Applying …

… laccase gene expression in the model fungus Aspergillus nidulans. … the laccase gene pslcc
from the white-rot fungus Pycnoporus … gene expression in filamentous fungal hosts, efficient …