… sensitivity of a published CFSE-based T-cell proliferation assay that demonstrated proinsulin
33-63 –specific CD4 + T cells … CFSE-based T-cell proliferation assay targeting rare antigen-…

… to enable detection of rare MA-specific T cells that would … of the autoantigen-specific T cell
repertoire while sparing T cells with … While naïve T cells are capable of extensive proliferation …

… data interpretation for results of proliferation assays. Here we … -off for detecting the response
of low frequency Ag-specific T cells, … assay is sensitive enough to detect an Ag-specific T cell …

… We provide evidences that a dye-based proliferation assay is as sensitive as other currently
… Detection of reactive T cells against autoAgs requires highly sensitive techniques due to the …

… T cells to drive their activation, proliferation, and effector cell differentiation and/or by differentiating
into autoantibody-secreting cells… repertoire that are as rare as 1 in 20 million cells (29)…

… Once these cells are isolated, in vitro proliferation assays and … The study of autoantigen-specific
B cells and a detailed … and characterize antigen-specific T cells, but to our knowledge …

… exclude the existence of autoantigen-specific CD8 + T cells recognizing unknown epitopes.
… Methotrexate treatment was shown to enhance the proliferation of circulating CD8 + T cells …

… pathophysiology, including rare anti-MuSK B cells and polyclonal … T cells were detected in
spleen and lymph nodes with … labeling, T cells were labeled with CTV Cell Proliferation Kit (…

… is particularly sensitive to rare antigen-specific T cells and … The ability to detect and
study antigen-specific T cells is … particularly challenging for autoantigen-specific CD4 + T-cell …

… approaches with following detection of activation, proliferation, or … The most sensitive method
to quantify γH2AX is indirect … If possible, autoantigen specific T cells should be monitored, …