Designing allele-specific competitive-extension PCR-based assays for high-throughput genotyping and gene characterization

R Kalendar, AV Shustov, I Akhmetollayev… - Frontiers in molecular …, 2022 - frontiersin.org
genotyping, each allele-specific primer is designed such that the penultimate base before the
primer’s … The tool allows design of custom FRET cassette reporter systems for fluorescence-…

Single nucleotide polymorphism genotyping using microarrays

T Pastinen - Microarrays and Microplates, 2023 - taylorfrancis.com
… and promise of DNA microarray technology for high-throughput genotyping and targeted …
allele-specific primer extension products. Each SNP is scored using two allele-specific probes …

Primer design for SNP genotyping based on allele-specific amplification—Application to organ transplantation pharmacogenomics

LA Tortajada-Genaro, R Puchades… - Journal of Pharmaceutical …, 2017 - Elsevier
Allele specific amplification in a homogeneous format or combined to microarray
hybridization are powerful approaches for SNP genotyping. However, …

High-throughput genotyping for cereal research and breeding

MW Ganal, J Plieske, A Hohmeyer, A Polley… - Applications of genetic …, 2019 - Elsevier
… Typically, such analyses are performed using microarray platforms from … allele-specific
forward primers are used for allele-specific amplification together with a single reverse primer. …

An innovative SNP genotyping method adapting to multiple platforms and throughputs

YM Long, WS Chao, GJ Ma, SS Xu, LL Qi - Theoretical and Applied …, 2017 - Springer
… , microarray-based and PCR-based, employed in genotyping of SNPs… -primer 2), and
40 nM of each of asymmetrically modified allele-specific primers (AMAS-primer 1 and AMAS-primer

Multiple genotyping based on multiplex PCR and microarray

XB Mou, Z Ali, B Li, TT Li, H Yi, HM Dong, NY He… - Chinese Chemical …, 2016 - Elsevier
… All the oligonucleotides including PCR primers and allele-specific probes used in this …
However this method has the potential to be used for high-throughput SNPs genotyping. …

Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection

ES Yamanaka, LA Tortajada-Genaro, Á Maquieira - Microchimica Acta, 2017 - Springer
… Most genomic findings have been obtained from high-throughputsystems is that
based on allele-specific (AS) amplification [7]. Polymerase chain reaction (PCR) uses primer

BatchPrimer3: A free web application for allele specific (SBE and allele flanking) primer design for SNPs genotyping in molecular diagnostics: A bioinformatics study

SK Ali, DAF Al-Koofee - Gene Reports, 2019 - Elsevier
… because it can be used in a wide variety of high-throughputprimer extension reaction
involves two types of primer design, single base extension primers and allele-specific primers

Methods of Genotyping

M Wjst, M Werner - Pharmacogenetics, 2016 - api.taylorfrancis.com
… genome, SNP genotyping requires high-throughput (HT) … to carry out allele-specific
hybridization on microarrays is the … based on allele-specific primer extension, allele-specific PCR…

Modified “Allele-specific qPCR” method for SNP genotyping based on FRET

R Kalendar, A Baidyussen, D Serikbay… - Frontiers in plant …, 2022 - frontiersin.org
… investigations into specific SNPs, to high-throughput microarray technologies involving …
genotyping method a more adaptable, universal, cost-effective, and high-throughput genotyping