Designing allele-specific competitive-extension PCR-based assays for high-throughput genotyping and gene characterization
R Kalendar, AV Shustov, I Akhmetollayev… - Frontiers in molecular …, 2022 - frontiersin.org
… genotyping, each allele-specific primer is designed such that the penultimate base before the
primer’s … The tool allows design of custom FRET cassette reporter systems for fluorescence-…
primer’s … The tool allows design of custom FRET cassette reporter systems for fluorescence-…
Single nucleotide polymorphism genotyping using microarrays
T Pastinen - Microarrays and Microplates, 2023 - taylorfrancis.com
… and promise of DNA microarray technology for high-throughput genotyping and targeted …
allele-specific primer extension products. Each SNP is scored using two allele-specific probes …
allele-specific primer extension products. Each SNP is scored using two allele-specific probes …
Primer design for SNP genotyping based on allele-specific amplification—Application to organ transplantation pharmacogenomics
LA Tortajada-Genaro, R Puchades… - Journal of Pharmaceutical …, 2017 - Elsevier
… Allele specific amplification in a homogeneous format or combined to microarray
hybridization are powerful approaches for SNP genotyping. However, …
hybridization are powerful approaches for SNP genotyping. However, …
High-throughput genotyping for cereal research and breeding
MW Ganal, J Plieske, A Hohmeyer, A Polley… - Applications of genetic …, 2019 - Elsevier
… Typically, such analyses are performed using microarray platforms from … allele-specific
forward primers are used for allele-specific amplification together with a single reverse primer. …
forward primers are used for allele-specific amplification together with a single reverse primer. …
An innovative SNP genotyping method adapting to multiple platforms and throughputs
… , microarray-based and PCR-based, employed in genotyping of SNPs… -primer 2), and
40 nM of each of asymmetrically modified allele-specific primers (AMAS-primer 1 and AMAS-primer …
40 nM of each of asymmetrically modified allele-specific primers (AMAS-primer 1 and AMAS-primer …
Multiple genotyping based on multiplex PCR and microarray
… All the oligonucleotides including PCR primers and allele-specific probes used in this …
However this method has the potential to be used for high-throughput SNPs genotyping. …
However this method has the potential to be used for high-throughput SNPs genotyping. …
Low-cost genotyping method based on allele-specific recombinase polymerase amplification and colorimetric microarray detection
ES Yamanaka, LA Tortajada-Genaro, Á Maquieira - Microchimica Acta, 2017 - Springer
… Most genomic findings have been obtained from high-throughput … systems is that
based on allele-specific (AS) amplification [7]. Polymerase chain reaction (PCR) uses primer …
based on allele-specific (AS) amplification [7]. Polymerase chain reaction (PCR) uses primer …
BatchPrimer3: A free web application for allele specific (SBE and allele flanking) primer design for SNPs genotyping in molecular diagnostics: A bioinformatics study
SK Ali, DAF Al-Koofee - Gene Reports, 2019 - Elsevier
… because it can be used in a wide variety of high-throughput … primer extension reaction
involves two types of primer design, single base extension primers and allele-specific primers…
involves two types of primer design, single base extension primers and allele-specific primers…
Methods of Genotyping
M Wjst, M Werner - Pharmacogenetics, 2016 - api.taylorfrancis.com
… genome, SNP genotyping requires high-throughput (HT) … to carry out allele-specific
hybridization on microarrays is the … based on allele-specific primer extension, allele-specific PCR…
hybridization on microarrays is the … based on allele-specific primer extension, allele-specific PCR…
Modified “Allele-specific qPCR” method for SNP genotyping based on FRET
R Kalendar, A Baidyussen, D Serikbay… - Frontiers in plant …, 2022 - frontiersin.org
… investigations into specific SNPs, to high-throughput microarray technologies involving …
genotyping method a more adaptable, universal, cost-effective, and high-throughput genotyping …
genotyping method a more adaptable, universal, cost-effective, and high-throughput genotyping …