Stabilities and instabilities in transgene expression

P Meyer - Transgenic plant research, 2022 - taylorfrancis.com
… to the presence of multiple copies of the transgene or homologous … its transcription, or by
post transcriptional processes that … High frequency, heat treatment-induced inactivation of the …

GC-rich coding sequences reduce transposon-like, small RNA-mediated transgene silencing

LV Sidorenko, T Lee, A Woosley, WA Moskal… - Nature plants, 2017 - nature.com
… or translational repression, resulting in post-transcriptional gene … In addition to the increased
frequency of methylated … biased LC-PUFA transgenes with repeated regulatory sequences …

[PDF][PDF] An RB-EZH2 complex mediates silencing of repetitive DNA sequences

CA Ishak, AE Marshall, DT Passos, CR White, SJ Kim… - Molecular cell, 2016 - cell.com
… Repetitive genomic regions include tandem sequence repeats and interspersed repeats,
such as … We anticipate that EZH2 inhibitors will cause widespread de-repression of repetitive …

Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability

J Kim, D Sturgill, AD Tran, DA Sinclair… - Nucleic acids …, 2016 - academic.oup.com
… evidence for persistent transcriptional repression in response to … Here, we generated a
transgenic mouse model, which … structures as well as canonical tandem repeats (57,58). …

Good News for Nuclear Transgene Expression in Chlamydomonas

M Schroda - Cells, 2019 - mdpi.com
Suppression of SET3 by RNAi released the transcriptional silencing of tandemly repeated
transgenes … indeed reported an increased frequency of high-level transgene expression in a …

[HTML][HTML] The road less traveled: strategies to enhance the frequency of homology-directed repair (HDR) for increased efficiency of CRISPR/Cas-mediated transgenesis

S Devkota - BMB reports, 2018 - ncbi.nlm.nih.gov
… in the reversal of HDR repression and increased HDR in G1 cells (52). … DNA, but in reverse
orientation. This strategy excludes the possibility of repeated cleavage of donor DNA after

Transgenic clustered regularly interspaced short palindromic repeat/Cas9-mediated viral gene targeting for antiviral therapy of Bombyx mori nucleopolyhedrovirus

S Chen, C Hou, H Bi, Y Wang, J Xu, M Li… - Journal of …, 2017 - Am Soc Microbiol
… ) genome DNA to promote virus clearance in silkworms. We demonstrate that transgenic
deletions in BmNPV genomes, and thus exhibit robust suppression of BmNPV proliferation. …

Propagation of Polycomb-repressed chromatin requires sequence-specific recruitment to DNA

F Laprell, K Finkl, J Müller - Science, 2017 - science.org
… and heritably maintained over multiple cell generations. For … the >dpp WE –TZ transgene
in the absence of PRE DNA. … gene repression after PRE excision in animals in which DNA

[PDF][PDF] An abundant class of non-coding DNA can prevent stochastic gene silencing in the C. elegans germline

C Frøkjær-Jensen, N Jain, L Hansen, MW Davis, Y Li… - Cell, 2016 - cell.com
… only transgenes with the longest introns (700–900 bps) were expressed at high frequency
-1 introns were repeated within transgenes; thus all three transgenes are relatively repetitive. (…

[PDF][PDF] Regulation of rice tillering by RNA-directed DNA methylation at miniature inverted-repeat transposable elements

L Xu, K Yuan, M Yuan, X Meng, M Chen, J Wu, J Li… - Molecular plant, 2020 - cell.com
… In this study, we find that RdDM suppresses rice tillering. One … tillering not through
transcriptional repression or activation of … All of the transgenic plants generated in this study are in …