[HTML][HTML] Small RNA populations revealed by blocking rRNA fragments in Drosophila melanogaster reproductive tissues

EK Fowler, I Mohorianu, DT Smith, T Dalmay… - PLoS …, 2018 - journals.plos.org
… or transfer RNA) present, potentially reflecting tissue-specific differences in biological functions.
For example, in Drosophila, … -mapping reads and challenge quantitative sRNA analyses. …

[HTML][HTML] Quantitative analysis of tRNA abundance and modifications by nanopore RNA sequencing

MC Lucas, LP Pryszcz, R Medina, I Milenkovic… - Nature …, 2023 - nature.com
tRNA populations that provides quantitative estimates of both … tRNA constructs (IVT Drosophila
melanogaster mitochondrial … mapped IVT tRNAs, it failed to map a single biological tRNA

Non-redundant tRNA reference sequences for deep sequencing analysis of tRNA abundance and epitranscriptomic RNA modifications

F Pichot, V Marchand, M Helm, Y Motorin - Genes, 2021 - mdpi.com
… For better efficiency, the tRNA population may be fractioned … and thus very ambiguous
mapping of tRNA reads, we foresee … Panels (A,B) show analysis of Drosophila melanogaster and …

tRNA 2′-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila

MT Angelova, DG Dimitrova, B Da Silva… - Nucleic Acids …, 2020 - academic.oup.com
… mass spectrometry and RiboMethSeq mapping revealed that … to yeast and humans, Drosophila
melanogaster has evolved two … For northern blotting analysis of tRNA, 10 μg of total RNA …

[HTML][HTML] Mitochondrial polyadenylation is a one-step process required for mRNA integrity and tRNA maturation

A Bratic, P Clemente, J Calvo-Garrido… - PLoS …, 2016 - journals.plos.org
… In this study we deleted the mitochondrial polymerase responsible for polyadenylation in the
fly, Drosophila melanogaster, … Our study also shows that polyadenylation does not regulate …

[HTML][HTML] Optimization of ribosome footprinting conditions for Ribo-Seq in human and Drosophila melanogaster tissue culture cells

K Douka, M Agapiou, I Birds, JL Aspden - Frontiers in Molecular …, 2022 - frontiersin.org
analysis, reads were globally mapped to 5′ and 3′ UTRs and coding regions (CDS) and
the mean number of reads that is mapped … originating from rRNA and tRNA were aligned and …

ELAC2/RNaseZ-linked cardiac hypertrophy in Drosophila melanogaster

E Migunova, J Theophilopoulos… - Disease Models & …, 2021 - journals.biologists.com
… This study provides first experimental evidence for the pathogenicity of … The most prevalent
form is HCM, with one in 500 people … ELAC2/RNaseZ endoribonuclease is essential for tRNA

Kc167, a widely used Drosophila cell line, contains an active primary piRNA pathway

N Vrettos, M Maragkakis, P Alexiou, Z Mourelatos - Rna, 2017 - rnajournal.cshlp.org
… , derived from Drosophila melanogaster embryos, expresses … Since most Kc167 piRNAs
are genic, they can be mapped … We performed this analysis independently for genic and tRNA-…

Transcriptome-wide analysis of pseudouridylation in Drosophila melanogaster

W Song, R Podicheti, DB Rusch, WD Tracey Jr - G3, 2023 - academic.oup.com
… Enzymatic Psi modification occurs on rRNA, snRNA, snoRNA, tRNA, and non-… Drosophila
melanogaster. Here, we optimized Psi-seq … The RNA samples we used for the mapping of …

[PDF][PDF] High-Throughput Assessment of small Open Reading Frame Translation in Drosophila melanogaster

MAS Mumtaz - 2016 - core.ac.uk
… This study was one of the first to highlight the fact that a SEP is translated and can perform …
on the genetic mapping of identified mutant phenotypes from a mutagen-treated population to …