… for long RNAs up-to-date. Here, we demonstrate that novel complementary-addressed SDSL
approach allows efficient spin labeling and following structural EPR studies of long RNAs. …

… is inherently more flexible, which makes some well-established techniques for structural
determination of proteins, such as X-ray crystallography (XRC) and cryo-electron microscopy (…

… determination of accurate distances in addition to orientations between two spin labels. This
chapter gives an overview of nucleic acids spin labeling … spin-labeling method for RNA is the …

… of biomacromolecules such as RNA. Here we introduce a spin labeling strategy based on
click … To determine the appropriate conformation for the two Gaussian components, the X-ray …

… problem associated with spin-labeling by chemical synthesis of … label Çm into RNA with
quantitative spin-labeling efficiency. … and their spin labeling efficiency was determined by spin-…

… determined by fluorescence titration studies. This spin-labeling approach is the first example
of … and site-directed spin-labeling that involves a native (unmodified) RNA. Pulsed electron-…

… We determined PRE rates obtained from aromatic 13 C … directed spin labeling methods and
their application for RNA, … Here, we apply a previously established noncovalent spin labeling …

… in nitroxide spin-labeling of nucleic acids for structure determination using rigid … spin-labeling
of duplex DNA and RNA containing abasic sites. No specific binding was observed for RNA…

… Depending on the desired positions for spin labeling within the RNA sequence of interest, …
Determine the concentration of cleaned spin-labeled RNA by measuring the absorption at …

… the ssRNA sequence only affected residues which do not display base-specific recognition.
… ], with spin labeling in different positions for EPR measurements or with spin labeling and …