Interest in silver nanoparticles (AgNPs) has recently increased due to their extensive and varied use, the biological synthesis of synthesis of AgNPs being safer than other approaches. The Kingdom of Saudi Arabia is one of the biggest producers of date palm, leading to the production of large amounts of waste date seeds. Here, the study evaluate the use of date seeds extract in the biosynthesis of AgNPs and to evaluate the antibacterial effect of the resultant nanoparticles (NPs) against pathogenic bacteria. A number of which were used here in order to characterize the synthesized particles, including UV-Vis absorption spectroscopy, transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). The results show that date seed extract can be used to synthesize AgNPs effectively. These AgNPs exhibit unique, small-size morphologies which proved to be antibacterial against pathogenic bacteria including Staphylococcus aureus, S. epidermidis, and Escherichia coli.