Use of a multiplex PCR assay to assess the presence of Treponema pallidum in mucocutaneous ulcerations in patients with suspected syphilis

PA Grange, A Jary, C Isnard, S Burrel… - Journal of clinical …, 2021 - Am Soc Microbiol
… Allplex genital ulcer real-time PCR multiplex assay for detecting Treponemareal-time
multiplex PCR assay for simultaneous detection of the genomes of Treponema pallidum, herpes …

Retrospective review of Treponema pallidum PCR and serology results: are both tests necessary?

A Brischetto, I Gassiep, D Whiley… - Journal of clinical …, 2018 - Am Soc Microbiol
… However, our results indicate that in our setting, the syphilis PCR test does not add
significant diagnostic value. … Clinical value of Treponema pallidum real-time PCR for …

A novel nested real-time polymerase chain reaction for Treponema pallidum DNA in syphilis biospecimens

Y Zhang, X Dai, Z Ren, H Lin, W Cao… - Sexually transmitted …, 2019 - journals.lww.com
… All procedures used in this study were approved by the medical ethics committee of the …
for real-time PCR, and we did not analyze syphilis diagnoses for positive DNA tests using NR-…

Cross‐sectional study of Treponema pallidum PCR in diagnosis of primary and secondary syphilis

M Costa‐Silva, D Coutinho… - International journal …, 2018 - Wiley Online Library
clinic with clinical suspicion of early syphilis. One or more specimens for the detection of Tp
by PCR testing … To detect Tp DNA, a published real time PCR assay targeting a sequence of …

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with …

E Vrbová, L Mikalová, L Grillová, P Pospíšilová… - PLoS …, 2020 - journals.plos.org
… of PCR detection of syphilis treponemes in clinical samples, this study shows that nested PCR
can improve syphilis diagnostics, … Clinical value of Treponema pallidum real-time PCR for …

Treponema pallidum ssp. pallidum identification by real-time PCR targetting the polA gene in paraffin-embedded samples positive by immunohistochemistry

A Gama, EM Carrillo-Casas… - … journal of STD & …, 2017 - journals.sagepub.com
… We found 125 formalin-fixed and paraffin-embedded biopsies between 1993 and 2015
by searching records containing the words syphilis or syphilitic as previous history, clinical, …

Quantified detection of Treponema pallidum DNA by PCR assays in urine and plasma of syphilis patients

C Wang, X Zheng, Z Guan, D Zou, X Gu… - Microbiology …, 2022 - Am Soc Microbiol
… Though the diagnosis of syphilis is mainly based on clinical manifestations combined with
… investigate the detection rate and loads of T. pallidum DNA in urine in syphilis patients at …

A New Specimen for Syphilis Diagnosis: Evidence by High Loads of Treponema pallidum DNA in Saliva

C Wang, Z Hu, X Zheng, M Ye, C Liao… - Clinical Infectious …, 2021 - academic.oup.com
Treponema pallidum DNA detection rates in saliva and plasma were 31.0% (9/29) and
51.7% (15/29) in primary syphilis (P = .11), 87.5% (63/72) and 61.1% (44/72) in secondary …

Laboratory diagnostic tools for syphilis: current status and future prospects

Y Luo, Y Xie, Y Xiao - Frontiers in cellular and infection microbiology, 2021 - frontiersin.org
… Therefore, a sensitive method for detecting T. pallidum is fundamental for the prompt
diagnosis of syphilis. Morphological … Clinical Value of Treponema pallidum Real-Time PCR for …

PCR detection for syphilis diagnosis: Status and prospects

C Zhou, X Zhang, W Zhang, J Duan… - Journal of clinical …, 2019 - Wiley Online Library
Syphilis, a re-emerging public health problem worldwide caused by Treponema pallidum
subsp pallidum (T… In this review, five studies about real-time PCR for syphilis diagnosis are …