Mapping the Plasticity of the Escherichia coli Genetic Code with Orthogonal Pair-Directed Sense Codon Reassignment

MA Schmitt, W Biddle, JD Fisk - Biochemistry, 2018 - ACS Publications
… machinery and the natural machinery in Escherichia coli. This report discusses 20 new,
related genetic codes, in which a targeted E. coli wobble codon is reassigned to tyrosine utilizing …

Reprogramming the genetic code

D de la Torre, JW Chin - Nature Reviews Genetics, 2021 - nature.com
Escherichia coli, where the principles of genetic code reprogramming are most developed.
Genetic code reprogramming requires codons… should realize codon reassignment for ncAA …

Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli

JM Ho, NM Reynolds, K Rivera, M Connolly… - ACS synthetic …, 2016 - ACS Publications
… avoids the problem of frameshifting, and a large number of sense codons are available for
reassignment due to the degeneracy of the genetic code. While sense codon reassignment

Expanding and reprogramming the genetic code

JW Chin - Nature, 2017 - nature.com
… The near-universal genetic code defines how 64 triplet codons … pair is orthogonal in Escherichia
coli and other bacteria 11 ; E. … steps in sense codon reassignment; synonymous codon

Rewriting the genetic code

T Mukai, MJ Lajoie, M Englert… - Annual review of …, 2017 - annualreviews.org
change the genetic code of their host cells for late gene expression. The full map of codon
reassignment … used to engineer the codon usage in the Escherichia coli genome. For serial …

Modification of orthogonal tRNAs: unexpected consequences for sense codon reassignment

W Biddle, MA Schmitt, JD Fisk - Nucleic acids research, 2016 - academic.oup.com
… Breaking the degeneracy of the genetic code via sense codon reassignment has emerged
as a way to incorporate multiple copies of multiple non-canonical amino acids into a protein of …

Refactoring the genetic code for increased evolvability

G Pines, JD Winkler, A Pines, RT Gill - MBio, 2017 - Am Soc Microbiol
Escherichia coli genes by using these generated codes to … of reassignments resulted in
another two genetic codes termed CMC (… coli genetic code, each codon can be converted into 6.1 …

Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii

REB Young, S Purton - Plant biotechnology journal, 2016 - Wiley Online Library
… , the assembly of expression cassettes in Escherichia coli when the gene product is toxic to
the … The existence of an unused codon in the C. reinhardtii chloroplast genetic code, together …

Measuring the tolerance of the genetic code to altered codon size

EA DeBenedictis, D Söll, KM Esvelt - Elife, 2022 - elifesciences.org
… tolerance of the Escherichia coli genetic code to tRNA mutations that increase codon size. …
readthrough assay to measure the fold change activity when mutations to bases 32, 37, and …

Xenomicrobiology: a roadmap for genetic code engineering

CG Acevedo‐Rocha, N Budisa - Microbial biotechnology, 2016 - Wiley Online Library
… of Escherichia coli), is only encoded by the single UGG codon, … , genetic code engineering
with ncAA by using amino acid auxotrophic strains, SCS and sense codon reassignment has …