… Here, we used covalent taxane-site ligands, including cyclostreptin, to gain … pore site 1,
which has been postulated to facilitate the entry of taxane-site ligands to their luminal binding site…

… , at the inner surface of the microtubule lumen [21]. It has a … and cyclostreptin bind covalently
to the binding site [60]. … taxoid 2α-benzoyltransferase, taxoid 10β-acetyltransferase and …

… is a marine macrolide that binds to the taxoid binding site on β-tubulin in a covalent fashion,
in … to the pore type I site, since zampanolide-saturated microtubules cannot bind cyclostreptin …

… accesses its binding site inside the microtubule lumen is by … III which is an inactive taxoid
precursor isolated from needles of … showed cyclostreptin covalently binds to microtubule with …

… Cyclostreptin (CyS, Fig. 1), the first drug known to … tubulin at the pore site both in vitro and
in cells, thereby producing the same cytotoxic effects as drugs binding to the luminal site [30], …

… On the other hand, cyclostreptin is known to bind covalently to both the MT pores and
luminal taxoid binding site 33,35 and strongly inhibits taxane binding. We quantified Flutax-2 …

… bind to a complex formed between tubulin and the stathmin-like protein RB3. RB3 sequesters
two longitudinally aligned tubulin … bind to its binding site in the context of atomic models of …

… to be a pocket adjacent to the taxoid-binding site on β-tubulin at the outer surface of the …
on the binding modes revealed that cyclostreptin covalently binds to a pore region of β-tubulin, …

… α-tubulin, or alternatively bind deeply in the β monomer, making no contacts with α-… tubulin
βIII isotype, as it is not affected by the Cys239 to Ser239 change in the colchicine binding site. …

… binding sites include a proposed imidazole site in β-tubulin … was shown to covalently bind
to H7 of β-tubulin via H230. In … can bind inside the lumen of MTs we polymerized tubulin in …