RNASeq methods for transcriptome analysis

R Hrdlickova, M Toloue, B Tian - Wiley Interdisciplinary …, 2017 - Wiley Online Library
… be selected using oligo-dT priming for reverse transcription (… is often desirable when a defined
group of genes is of interest… of regions of interest by hybridization of RNA-Seq libraries to …

Improved definition of the mouse transcriptome via targeted RNA sequencing

G Bussotti, T Leonardi, MB Clark, TR Mercer… - Genome …, 2016 - genome.cshlp.org
… coverage restrictions usually imposed by RNA-seq and the joining of previously … transcription
within these regions using signal tracks (.wig) retrieved from the ENCODE/LICR RNA-seq

[HTML][HTML] A survey of best practices for RNA-seq data analysis

A Conesa, P Madrigal, S Tarazona, D Gomez-Cabrero… - Genome biology, 2016 - Springer
… This is achieved by first defining a good experimental design… used random hexamer priming
to reverse-transcribe poly(A)-… to a limited number of identical regions equally well (‘multi-…

[HTML][HTML] RNA-Seq perspectives to improve clinical diagnosis

G Marco-Puche, S Lois, J Benítez, JC Trivino - Frontiers in genetics, 2019 - frontiersin.org
… ASpedia provides genomic annotations extracted from DNA, RNA and proteins, transcription
In addition, it is necessary to define the laboratory and bioinformatics parameters and tools …

[HTML][HTML] Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis

SME Sahraeian, M Mohiyuddin, R Sebra… - Nature …, 2017 - nature.com
… FDR was then defined as the proportion of reported RNA … , the strands of the RNA-seq reads
were extracted using the … to, excluding the regions with bidirectional transcription. Ensembl, …

RNA sequencing (RNA-Seq) and its application in ovarian cancer

J Wang, DC Dean, FJ Hornicek, H Shi, Z Duan - Gynecologic oncology, 2019 - Elsevier
… ascertaining pathological origin, and defining the aberrant genes and … RNA-Seq can also
detect transcription boundaries with a … expression, and SNPs in the transcribed regions [15,24]. …

[HTML][HTML] Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq

L Chappell, P Ross, L Orchard, TJ Russell, TD Otto… - BMC genomics, 2020 - Springer
… We also find that transcription from bidirectional promoters … by defining the position at which
continuous RNA-seq coverage … maximise the signal for defining this region. Like DAFT-seq, …

[HTML][HTML] Measure transcript integrity using RNA-seq data

L Wang, J Nie, H Sicotte, Y Li, JE Eckel-Passow… - BMC …, 2016 - Springer
… a dominant role in determining the fragment size whereas … area in the region of 18S
and 28S compared to the total areafrom 5′ end (transcription start site) to 3′ end (transcription

Optimizing RNA-Seq mapping with STAR

A Dobin, TR Gingeras - Data mining techniques for the life sciences, 2016 - Springer
… Sequencing of transcribed RNA molecules (RNA-seq ) is an … , requiring mapping to
noncontiguous regions of the genome. … parameter which defines the number of bases taken from

[HTML][HTML] Prediction and quantification of splice events from RNA-seq data

LD Goldstein, Y Cao, G Pau, M Lawrence, TD Wu… - PloS one, 2016 - journals.plos.org
… (1) Methods for the quantification of defined splice events (eg … to exonic regions and splice
junctions, directed from the 5′ to … define a genome-wide splice graph for transcribed genomic …