Clinical Impact of Polymerase Chain Reaction–Based Aspergillus and Azole Resistance Detection in Invasive Aspergillosis: A Prospective Multicenter Study
S Huygens, A Dunbar, JB Buil… - Clinical Infectious …, 2023 - academic.oup.com
… Aspergillus was cultured from BALf in 7.5% (24 of 323) of patients… Aspergillus fumigatus
was cultured in 21, while Aspergillus flavus, Aspergillus terreus, and Aspergillus niger were each …
was cultured in 21, while Aspergillus flavus, Aspergillus terreus, and Aspergillus niger were each …
PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar lavage: a multicentre validation of the AsperGenius assay® in 201 patients …
GM Chong, MT van der Beek… - Journal of …, 2016 - academic.oup.com
… differentiated WT from Aspergillus fumigatus with RAMs, including in culture-negative BAL
samples. Most importantly, detection of RAMs was associated with azole treatment failure. …
samples. Most importantly, detection of RAMs was associated with azole treatment failure. …
Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of Aspergillus species in the Human Airway
TY Poh, NABM Ali, LLY Chan, PY Tiew… - International journal of …, 2020 - mdpi.com
… (as compared to qPCR) in detecting Aspergillus species in the normal and diseased airway
… using the 18S ITS sequence of Aspergillus fumigatus (accession number NR_121481) and …
… using the 18S ITS sequence of Aspergillus fumigatus (accession number NR_121481) and …
(New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples
… for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR)
to detect mutations to the Cyp51A protein, have been developed which are able to detect most …
to detect mutations to the Cyp51A protein, have been developed which are able to detect most …
Diagnostic performance of real‐time polymerase chain reaction assay on blood for invasive aspergillosis and mucormycosis
H Seo, JY Kim, HJ Son, J Jung, MJ Kim, YP Chong… - Mycoses, 2021 - Wiley Online Library
… of the RT-PCR assays: the species Aspergillus fumigatus, A. flavus, A. … detected Aspergillus
PCR in blood samples from 25 of 46 patients with proven or probable invasive aspergillosis …
PCR in blood samples from 25 of 46 patients with proven or probable invasive aspergillosis …
Optimization of a real-time PCR assay for identification of Aspergillus fumigatus and Aspergillus Niger from flour samples: comparison of phenotypic and genotypic …
S Ghafori, R Habibipour, S Bayat - Gene Reports, 2021 - Elsevier
… The polymerase chain reaction (PCR) is a sensitive and rapid method for identifying food-stuff
contaminations; however, the target gene's amplification cannot be observed in real-time (…
contaminations; however, the target gene's amplification cannot be observed in real-time (…
A simple and low cost tetra-primer ARMS-PCR method for detection triazole-resistant Aspergillus fumigatus
S Mahmoudi, H Badali, S Rezaie, A Azarnezhad… - Molecular biology …, 2019 - Springer
… -polymerase chain reaction (ARMS-PCR) is a simple and economical technique for single
nucleotide polymorphisms (SNP) detection [19]… detection of the TR 34 /L98H mutation in the A. …
nucleotide polymorphisms (SNP) detection [19]… detection of the TR 34 /L98H mutation in the A. …
High-Frequency Direct Detection of Triazole Resistance in Aspergillus fumigatus from Patients with Chronic Pulmonary Fungal Diseases in India
… real-time polymerase chain reaction (PCR) assay to detect TR 34 /L98H, and TR 46 /T289A/Y121F
mutations and the AsperGenius ® G54/M220 RUO PCR assay to detect G54/M220 …
mutations and the AsperGenius ® G54/M220 RUO PCR assay to detect G54/M220 …
Standardization of a two-step real-time polymerase chain reaction based method for species-specific detection of medically important Aspergillus species
… based reports suggest prevalence of Aspergillus fumigatus is more common than any other
… in accurate identification of Aspergillus, we have tested all four Aspergillus isolates against …
… in accurate identification of Aspergillus, we have tested all four Aspergillus isolates against …
Culture-based methods and molecular tools for azole-resistant Aspergillus fumigatus detection in a Belgian university hospital
I Montesinos, MA Argudin, M Hites… - Journal of clinical …, 2017 - Am Soc Microbiol
… PCR-based detection of Aspergillus fumigatus Cyp51A mutations on bronchoalveolar …
disease suspected for invasive aspergillosis. J Antimicrob Chemother 71:3528–3535. …
disease suspected for invasive aspergillosis. J Antimicrob Chemother 71:3528–3535. …