The work describes a biofilm-based soluble sulphate reduction system, which can treat up to 1600ppm of solublesulphate within 3.5 hours of incubation to dischargelevelunder ambient condition using a well-characterized sulphate-reducing bacterial (SRB) consortium. Thissystemensuresthetreatmentof 1509litresofsulphatesolutionin24hours using a 220-litre bioreactor. Performance of the system during series operation was compromised, indicating the presence of inhibitorinsolutionata toxiclevel. A single unit bioreactor would be the ideal configuration for this consortium. Modified designs of bioreactors were tested for optimization of the process using response surface methodology (RSM), where the system could function optimally at an initial sulphate concentration of 1250ppm with a flow rate of 1.8 litre/hour. The time course of sulphate reduction yielded a parabolic profile (with coefficient of determination r2= 0.99 and p value< 0.05). The rate of sulphate reduction was found to be independent of seasonal variation as well as the specific design characteristic.