… The expression of heterologous genes into these regions to use … with the S ectodomain of
the mouse hepatitis virus (MHV) … a pathogenic strains like the IBV strains M41, H120 and QX […

… , novel strains and variants of infectious bronchitis virus (IBV) … by the two genes, ORF1a and
ORF1b, which are expressed as … Massachusetts type strain (95.2% similarity to strain H120) …

… to the live attenuated vaccine strains used in the regions, or are … S2 ectodomain. Asparagine
(N)-linked glycosylation sites in … All claims expressed in this article are solely those of the …

… virus titer as recombinant and wild type IBV strain H52. Thus, … attenuated IBV vaccine, H120,
was developed in the 1960s … rIBVs were generated by introducing the IBV spike ectodomain …

… The larger part of the ectodomain is formed by the S1 subunit … To identify the IBV strain, the
S1 gene amplicons must be … dynamics similar to those exhibited by the parental H120 strain…

… in a recombinant virus system are deficient in viral release … (D) Western blot analysis of viral
protein expression in Vero … coli strain BL21(DE3) CodonPlus for protein expression. Cells …

… Commercial Mass-type H-120 and Ma5 as well as other Mass … differ in strain of origin and
method of development (eg, … vaccine viral load and expression of genes associated with …

… on virus strains, reproductive and urogenital tracts. … trimeric recombinant S-ectodomain
proteins with S1 domains … the attenuated Mass serotype H120 vaccine strain bind to tracheal …

… The S2 subunit consists of a narrow stalk ectodomain, a short … the North African variant strains
and was different from the H120 (GI-… Genetic analysis of MENA IBV strains based on HVR3 …

… The strategy adopted by coronaviruses in gene expression … mandatory to detect and
characterize IBV strains. The most … For instance, H120-vaccinated animals showed a reduced viral …