… The duplex real-time PCR assay developed in this study can … rickettsia infection simultaneously
by performing 1 real-time … 5′ nuclease real-time PCR for spotted fever and typhus group …

… singleplex realtime PCR on … typhus cases in Orange and Los Angeles Counties and the
concomitant need to evaluate more fleas, we were prompted to develop a duplex real time-PCR …

… developed a quantitative, multiplex 5′-nuclease quantitative … CFX384 PCR instrument) or
separate triplex and duplex assays (… evaluation of real-time PCR assays for the detection of …

… fever group (SFG), typhus group (TG), Rickettsia bellii group, and … Real-time PCR (RT-PCR)
is a rapid and sensitive method, … The assay is combined in a duplex RT-PCR also amplifying …

… develop reliable and easy detection method for both these pathogens. We made a new
hydrolysis probe based duplex Real-Time PCR … groups: typhus group (TG), spotted fever group (…

… was to develop and validate a duplex PCR for detection of … , and other ticks positive to gltA
and ompB genes of Rickettsia sp. … Among those, real-time PCR strategies represent the new …

… prowazekii represent the typhus group (TG) of rickettsiae and … We developed and evaluated
two new qPCRs by … of real-time pcr assays for the detection of Orientia tsutsugamushi and …

… detection tools that are currently available or in development … Rickettsia typhi, which are
members of the typhus group (TG) … specific and sensitive detection of Rickettsia spp. Target …

… Both classic louse-borne and flea-borne typhus rickettsiae … Mammals such as Rattus spp.
serve as the main reservoirs … Thus, one purpose of the present study was to develop this …

… to distinguish spotted fever group and typhus group Rickettsia spp. have been developed
and used for … A prospective evaluation of real-time PCR assays for the detection of Orientia …