An eight-plex immunoassay for Group A streptococcus serology and vaccine development

AL Whitcombe, F Han, SM McAlister… - Journal of …, 2022 - Elsevier
develop a multiplex beadbased immunoassay that can detect and quantify antibody responses
Quantification of IgG antibodies and standardisation between assay runs was achieved …

Novel Multiplex Immunoassays for Quantification of IgG against Group B Streptococcus Capsular Polysaccharides in Human Sera

G Buffi, B Galletti, M Stella, D Proietti, E Balducci… - Msphere, 2019 - Am Soc Microbiol
… for the simultaneous determination of antibody responses to … Seventeen of the analyzed
sera showed mean fluorescence … to develop two microsphere multiplex immunoassays based …

Development and Evaluation of a New Triplex Immunoassay That Detects Group A Streptococcus Antibodies for the Diagnosis of Rheumatic Fever

AL Whitcombe, P Hanson-Manful, S Jack… - Journal of Clinical …, 2020 - Am Soc Microbiol
… in antibody response. Accordingly, our objective was to … design and optimize the multiplex
immunoassay technology for the … Forty-five of these participants were excluded from analysis, …

Development of an extended-specificity multiplex immunoassay for detection of streptococcus pneumoniae serotype-specific antigen in urine by use of human …

SD Eletu, CL Sheppard, E Thomas… - Clinical and Vaccine …, 2017 - Am Soc Microbiol
antibody responses to pneumococcal serotypes (21, 36). In 2011, our laboratory developed
a sensitive and specific multiplex immunoassayAnalysis of the fluorescence intensity (FI) …

The novel group A Streptococcus antigen SpnA combined with bead-based immunoassay technology improves streptococcal serology for the diagnosis of acute …

P Hanson-Manful, AL Whitcombe, PG Young… - Journal of Infection, 2018 - Elsevier
… of the antibody response, that is, the rate at which antibody … the determination of streptococcal
titres to be multiplexed. … A multiplex bead-based immunoassay has been developed

[HTML][HTML] Development and application of a multiplex assay for the simultaneous measurement of antibody responses elicited by common childhood vaccines

HL Itell, EP McGuire, P Muresan, CK Cunningham… - Vaccine, 2018 - Elsevier
antibody responses against multiple vaccines with small sample volumes, we developed a
Luminex multiplex … Because our analysis was limited by the small size of the placebo groups

Developing a salivary antibody multiplex immunoassay to measure human exposure to environmental pathogens

SAJ Augustine, TN Eason, KJ Simmons… - JoVE (Journal of …, 2016 - jove.com
… the development and analysis of a bead-based multiplex … ,3 antibody responses to these
pathogens using a bead-based … to improve sensitivity and to increase median fluorescent signal …

Development and Validation of 13-plex Luminex-Based Assay for Measuring Human Serum Antibodies to Streptococcus pneumoniae Capsular Polysaccharides

D Pavliakova, PC Giardina, S Moghazeh, S Sebastian… - Msphere, 2018 - Am Soc Microbiol
… pediatric population make analysis of antibody responses to the … Multiplexed fluorescent
microsphere-based assays that have … the development and validation of a multiplex Luminex-…

Interlaboratory comparison of a multiplex immunoassay that measures human serum IgG antibodies against six-group B streptococcus polysaccharides

K Le Doare, MA Gaylord, AS Anderson… - Human Vaccines & …, 2024 - Taylor & Francis
… assay may be applied to vaccine development and could be used to … Luminex-based
immunoassay that was developed to quantify … The funders had no role in the design, analysis, or …

Development and validation of a robust multiplex serological assay to quantify antibodies specific to pertussis antigens

G Rajam, G Carlone, E Kim, J Choi, S Paulos, SH Park… - Biologicals, 2019 - Elsevier
… Although there is no definitive threshold of antibody responseantibody density) is expressed
as the median fluorescent … for the simultaneous quantification of IgG antibodies specific …