Enhancing the in vitro and in vivo stabilities of polymeric nucleic acid delivery nanosystems

Y Wang, M Ye, R Xie, S Gong - Bioconjugate chemistry, 2018 - ACS Publications
… (eg, polymer molecular weight and structure, and N/P ratio) and … with the serum proteins, and
physiological ionic strength). In … Polyplexes exhibiting insufficient stability in the presence of …

Fluorescence Resonance Energy Transfer (FRET)-Based Analysis of Lipoplexes

E Pozharski - Liposomes: Methods and Protocols, 2017 - Springer
… three applications of FRET technique to analyze structural and … is compensated without much
effect on lipoplex formation. … Presence of free DNA increases the apparent FRET efficiency, …

Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide

SK Bhangu, L Mummolo, S Fernandes… - Advanced Functional …, 2024 - Wiley Online Library
… to polyplexes and lipoplexes following endosomal escape. [ 5 ] … curves at different pH and
low ionic strength (14 mm NaCl, … by both contact and FRET effects (ie, FRET triplex = 100%) a …

Disruption of Endosomal Membrane by Cationic Vectors Drives Endosomal Release and Enables Successful Gene Delivery

S Vaidyanathan - 2016 - deepblue.lib.umich.edu
… OMB FRET in the presence of polymers. .....................................… (OMB) labeled with a FRET
dye pair to distinguish intact DNA … that also have a rich nanoscale structure, we were interested …

Smart delivery systems for cancer treatment using advanced liposome formulations: fabrication, spectroscopic characterization and cell internalization studies

R Ozolina - 2016 - trepo.tuni.fi
… factors that affect rate and probability of contact due to short interaction distance. FRET on the
FRET with the size of the molecules, one can see that FRET is a through-space interaction. …

Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Förster Resonance Energy Transfer Analysis

D Zhang, Y Song, Y Wang, X Liu, W Yu, X Ma - Journal of Pharmaceutical …, 2016 - Elsevier
… In this study, the purpose is to adopt FRET technique to … presence of lysozyme influences
size and zeta potential of NPs. … the same condition (pH and ionic strength), so that FE change …

Molecular beacon strategies for sensing purpose

N Bidar, M Amini, F Oroojalian, B Baradaran… - TrAC Trends in …, 2021 - Elsevier
… Pb 2+ ions in biological samples such as human serum samples and water with … existence
of K + , the sensor created a firm G-quadruplex with the two dyes in near proximity to let FRET

Ionic strength sensing in living cells

B Liu, B Poolman, AJ Boersma - ACS chemical biology, 2017 - ACS Publications
… energy transfer (FRET). These probes allow observation of spatiotemporal changes in the …
of serum-free DMEM, according to the manufacturer’s instructions. A total of 25 μL of lipoplex

[PDF][PDF] A biophysical approach to mitochondrial gene therapy: gemini surfactant-based colloidal vectors

RS Bessa - 2016 - repositorio-aberto.up.pt
… and cytotoxicity, varying significantly with the presence of helper lipids. This work is a …
effect, called the helper lipids(39). These mixed systems are usually referred to as lipoplexes

Mechanistic Study of DNA Delivery from Self-Assembled Nanolayered Films

S Wang - 2022 - dspace.mit.edu
… Solution pH and ionic strength-dependent changes in film … nucleases that degrade DNA,
serum proteins that mark DNA for … interactions resulting in lipoplexes with net positive charge, …