Enhancing the in vitro and in vivo stabilities of polymeric nucleic acid delivery nanosystems
… (eg, polymer molecular weight and structure, and N/P ratio) and … with the serum proteins, and
physiological ionic strength). In … Polyplexes exhibiting insufficient stability in the presence of …
physiological ionic strength). In … Polyplexes exhibiting insufficient stability in the presence of …
Fluorescence Resonance Energy Transfer (FRET)-Based Analysis of Lipoplexes
E Pozharski - Liposomes: Methods and Protocols, 2017 - Springer
… three applications of FRET technique to analyze structural and … is compensated without much
effect on lipoplex formation. … Presence of free DNA increases the apparent FRET efficiency, …
effect on lipoplex formation. … Presence of free DNA increases the apparent FRET efficiency, …
Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide
SK Bhangu, L Mummolo, S Fernandes… - Advanced Functional …, 2024 - Wiley Online Library
… to polyplexes and lipoplexes following endosomal escape. [ 5 ] … curves at different pH and
low ionic strength (14 mm NaCl, … by both contact and FRET effects (ie, FRET triplex = 100%) a …
low ionic strength (14 mm NaCl, … by both contact and FRET effects (ie, FRET triplex = 100%) a …
Disruption of Endosomal Membrane by Cationic Vectors Drives Endosomal Release and Enables Successful Gene Delivery
S Vaidyanathan - 2016 - deepblue.lib.umich.edu
… OMB FRET in the presence of polymers. .....................................… (OMB) labeled with a FRET
dye pair to distinguish intact DNA … that also have a rich nanoscale structure, we were interested …
dye pair to distinguish intact DNA … that also have a rich nanoscale structure, we were interested …
Smart delivery systems for cancer treatment using advanced liposome formulations: fabrication, spectroscopic characterization and cell internalization studies
R Ozolina - 2016 - trepo.tuni.fi
… factors that affect rate and probability of contact due to short interaction distance. FRET on the
… FRET with the size of the molecules, one can see that FRET is a through-space interaction. …
… FRET with the size of the molecules, one can see that FRET is a through-space interaction. …
Insight of In Vitro Small-Interfering RNA Release From Chitosan Nanoparticles Under Enzymolysis With Förster Resonance Energy Transfer Analysis
D Zhang, Y Song, Y Wang, X Liu, W Yu, X Ma - Journal of Pharmaceutical …, 2016 - Elsevier
… In this study, the purpose is to adopt FRET technique to … presence of lysozyme influences
size and zeta potential of NPs. … the same condition (pH and ionic strength), so that FE change …
size and zeta potential of NPs. … the same condition (pH and ionic strength), so that FE change …
Molecular beacon strategies for sensing purpose
… Pb 2+ ions in biological samples such as human serum samples and water with … existence
of K + , the sensor created a firm G-quadruplex with the two dyes in near proximity to let FRET …
of K + , the sensor created a firm G-quadruplex with the two dyes in near proximity to let FRET …
Ionic strength sensing in living cells
… energy transfer (FRET). These probes allow observation of spatiotemporal changes in the …
of serum-free DMEM, according to the manufacturer’s instructions. A total of 25 μL of lipoplex …
of serum-free DMEM, according to the manufacturer’s instructions. A total of 25 μL of lipoplex …
[PDF][PDF] A biophysical approach to mitochondrial gene therapy: gemini surfactant-based colloidal vectors
RS Bessa - 2016 - repositorio-aberto.up.pt
… and cytotoxicity, varying significantly with the presence of helper lipids. This work is a …
effect, called the helper lipids(39). These mixed systems are usually referred to as lipoplexes…
effect, called the helper lipids(39). These mixed systems are usually referred to as lipoplexes…
Mechanistic Study of DNA Delivery from Self-Assembled Nanolayered Films
S Wang - 2022 - dspace.mit.edu
… Solution pH and ionic strength-dependent changes in film … nucleases that degrade DNA,
serum proteins that mark DNA for … interactions resulting in lipoplexes with net positive charge, …
serum proteins that mark DNA for … interactions resulting in lipoplexes with net positive charge, …