… Proteins For mouse immunization, human CD27 ECD-Fc protein was purchased from
R&D systems. For binding assays, amino acids 2–122 of the CD27 ECD (corresponding to …

… of in vitro refolded empty murine class I proteins (76). … studies and/or NMR and X-ray
crystallography. In the non-catalyzed … Solutions are most likely to come from protein engineering …

… gp33(KAVYNFATM) presented by H-2D b and complexed with mouse β 2 m (mβ 2 m) or hβ
2 m … during X-ray diffraction. (F) The crystal is formed from Ptal-N*01:01/HeV1 complex which …

… I major histocompatibility complex (MHC1) [14]. MHC1 presents antigenic peptide fragments
to the T cell receptor (TCR) in complex with … Codons for both ORFs were engineered by …

… , X-ray crystallographic, mutational, and developmental studies. For example, random
examination of mouse T … MHC mutations were cloned by overlapping primers using engineered …

… Specifically, we engineered genetic sequences with … X-ray diffraction experiments, the
intensity of the diffraction spots … with cell surface markers or mouse IgG2a isotype control in 1% …

… NK cell inhibitory receptors ligate human lymphocyte antigen (HLA) or major histocompatibility
complex (MHC) class I molecules expressed on healthy cells as self. Conversely, cells …

… Major Histocompatibility Complex (MHC) class I molecules … useful when engineering new
TCRs to fight diseases. … experimental data obtained through X-ray crystallography and/or NMR …

… on TCR gene-engineered primary human T cells, we did not observe a significant effect of β
2 m … (8) X-ray crystallographic analyses of TCR–pMHC complexes have revealed a general …

… Alternating selections against mouse and human serum albumins did not yield cross … for
chemical and recombinant protein engineering. In our antibody engineering experiments, we …