The genetic and phenotypic variability among 22 Jordanian faba bean (Vicia faba L.) accessions were explored. Fifteen horticultural traits were evaluated including Biological weight, Days to flowering, Grain yield, Plant height, Highest lowest pod, Number of primary branches, Pod length, Pod width, Pod weight, days to filling, pod number node-¹, Pod number plant-¹, number of secondary branches, seeds number per pod, 100 seed weight. Statistical analysis indicated significant variability among accessions for days to flowering, pod width, pod weight and grain yield. Genetic relationships among twenty two accessions were constructed using twelve EST-SSR primer pairs and eight AFLP primer combinations. The UPGMA results, based on phenotypical data, were able to divide the accessions into 3 groups. In contrast, the UPGMA results, based on DNA markers data, divided the accessions into five groups with Dice similarity coefficient of 0.62. This obtained information on the genetic and phenotypic diversity within gene pools in breeding programs could help avoid genetic erosion.