Large-scale collection of full-length cDNA and transcriptome analysis in Hevea brasiliensis
Y Makita, KK Ng, G Veera Singham… - DNA …, 2017 - academic.oup.com
… cultivar and sequenced around 20,000 clones by the Sanger method and over 15,000 contigs
… q-value ≤ 0.05 in biological function: generation of precursor metabolites and energy (GO:…
… q-value ≤ 0.05 in biological function: generation of precursor metabolites and energy (GO:…
[HTML][HTML] A comprehensive mouse transcriptomic BodyMap across 17 tissues by RNA-seq
… The mouse shares more than 15,000 protein-coding genes … With the advances of next-generation
sequencing … 12 , they constructed full-length cDNA libraries from various mouse organs …
sequencing … 12 , they constructed full-length cDNA libraries from various mouse organs …
[HTML][HTML] Full-Length Transcriptome and Gene Expression Analysis of Different Ovis aries Adipose Tissues Reveals Transcript Variants Involved in Lipid Biosynthesis
L An, Y Pan, M Yuan, Z Wen, L Qiao, W Wang, J Liu… - Animals, 2023 - mdpi.com
… PCR to obtain sufficient cDNA for short-length sequencing. All … , maximum subread length
= 15,000, minimum number of passes … of second-generation sequencing, the full-length reads …
= 15,000, minimum number of passes … of second-generation sequencing, the full-length reads …
[HTML][HTML] High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for …
KG Mitchell, B Gong, SS Hunter, D Burkart-Waco… - Scientific Reports, 2023 - nature.com
… The cDNA was subsequently PCR-amplified with a cocktail of four … not correspond to the
full-length coding regions of mouse V L … This contains ≈ 15,000 sequences for rearranged, …
full-length coding regions of mouse V L … This contains ≈ 15,000 sequences for rearranged, …
Genomic Characterization of Human Long Noncoding RNAs
J Lagarde - 2020 - diposit.ub.edu
… called Next-Generation Sequencing (NGS) methods more than … does not provide fulllength
cDNA sequences, and transcripts … genes between 15,000 and 18,000118. Gene counts in the …
cDNA sequences, and transcripts … genes between 15,000 and 18,000118. Gene counts in the …
[HTML][HTML] Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A
… After centrifugation at 15,000g for 15 min, cell debris was … observed in this study appeared
in field strain sequences. An … mice, but not in the natural host cells and animals. In this …
in field strain sequences. An … mice, but not in the natural host cells and animals. In this …
[HTML][HTML] Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm
… -read/second-generation sequencing) can only attain short … on ice, the precipitate was
pelleted at 15,000 g, 4 C, 30 min. … with the mouse assembly to identify the full-length long reads, …
pelleted at 15,000 g, 4 C, 30 min. … with the mouse assembly to identify the full-length long reads, …
[HTML][HTML] Poly (A)-seq: A method for direct sequencing and analysis of the transcriptomic poly (A)-tails
F Yu, Y Zhang, C Cheng, W Wang, Z Zhou, W Rang… - PloS one, 2020 - journals.plos.org
… With the arrival of the era of next-generation sequencing (… TAIL-seq [14], and for mouse NIH
3T3 cells the median poly(A) … poly(A) sequence information from the first cDNA strand of Poly(…
3T3 cells the median poly(A) … poly(A) sequence information from the first cDNA strand of Poly(…
Analysis of paramyxovirus transcription and replication by high-throughput sequencing
EB Wignall-Fleming, DJ Hughes, S Vattipally… - Journal of …, 2019 - Am Soc Microbiol
… genomes that are typically 15,000 to 19,000 nucleotides (nt) … first nucleotide of the IG region
to be important in generating … This produces a full-length, faithful, positive-sense copy of the …
to be important in generating … This produces a full-length, faithful, positive-sense copy of the …
Next-generation sequencing and emerging technologies
… sequencing include WGS, targeted sequencing, full-length … to be converted to cDNA before
sequencing, thereby losing … in primary mouse lymphoma and human leukemia samples.[68] …
sequencing, thereby losing … in primary mouse lymphoma and human leukemia samples.[68] …