Genome hunting of carbonyl reductases from Candida glabrata for efficient preparation of chiral secondary alcohols
G Xu, Y Zhang, Y Wang, Y Ni - Bioresource technology, 2018 - Elsevier
In this work, genome hunting strategy was adopted in screening for reductases from
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli
BL21 (DE3). A substrate library containing 32 substrates was established for
characterization of each reductase by average specific activity and Shannon-Wiener index.
Among them, Cg26 was identified with the highest efficiency and wider substrate spectrum
in the reduction of prochiral ketones, with average activity and Shannon-Wiener index of …
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli
BL21 (DE3). A substrate library containing 32 substrates was established for
characterization of each reductase by average specific activity and Shannon-Wiener index.
Among them, Cg26 was identified with the highest efficiency and wider substrate spectrum
in the reduction of prochiral ketones, with average activity and Shannon-Wiener index of …
Genome hunting of carbonyl reductases from Candida glabrata for efficient preparation of chiral secondary alcohols.
XGC Xu GuoChao, ZYP Zhang YaPing, WY Wang Yue… - 2018 - cabidigitallibrary.org
In this work, genome hunting strategy was adopted in screening for reductases from
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli
BL21 (DE3). A substrate library containing 32 substrates was established for
characterization of each reductase by average specific activity and Shannon-Wiener index.
Among them, Cg26 was identified with the highest efficiency and wider substrate spectrum
in the reduction of prochiral ketones, with average activity and Shannon-Wiener index of …
Candida glabrata. A total of 37 putative reductases were successfully expressed in E. coli
BL21 (DE3). A substrate library containing 32 substrates was established for
characterization of each reductase by average specific activity and Shannon-Wiener index.
Among them, Cg26 was identified with the highest efficiency and wider substrate spectrum
in the reduction of prochiral ketones, with average activity and Shannon-Wiener index of …
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