[PDF][PDF] Response Surface Optimization of the Expression Conditions for Synthetic Human Interferon α-2b Gene in Escherichia coli.

ZE Samarin, S Abolghasemi, E Dehnavi… - Indian Journal of …, 2018 - researchgate.net
… of recombinant human interferon α-2b remains to be addressed. In this study, the expression
of synthetic human interferon α-2b gene in Escherichia coli … including the onset of glucose

… miniature shaken bioreactor system as a scale-down model for process development of production of therapeutic alpha-interferon2b by recombinant Escherichia coli

JS Tan, S Abbasiliasi, S Kadkhodaei, YJ Tam… - BMC microbiology, 2018 - Springer
… of α-IFN2b production by recombinant Escherichia coli. The extraction performance of α-IFN2b …
to enhance growth rate, which resulted in higher consumption of glucose and induction of …

[PDF][PDF] Production, Purification and Characterization of IFN-α2b in Escherichia coli

EHA Mageed, FH Shary, AA Almayahi - International Journal of Drug …, 2022 - academia.edu
… research are expressed using recombinant DNA technology, … Escherichia coli the most
widely used prokaryotic systems … for the production of recombinant protein in escherichia coli. …

Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations

SJ Davarpanah - Protein Expression and Purification, 2022 - Elsevier
… method to refold IFN-α-2b at higher protein concentrations with good recovery. … of recombinant
interferon alpha-2b (IFNα-2b) expressed as insoluble inclusion bodies in Escherichia coli. …

Interferon-based biopharmaceuticals: Overview on the production, purification, and formulation

LS Castro, GS Lobo, P Pereira, MG Freire, MC Neves… - Vaccines, 2021 - mdpi.com
… formulations are produced mainly using Escherichia coli (E. … readily metabolizable carbon
sources such as glucose [39]. On … of therapeutic IFNs in Escherichia coli recombinant systems. …

Recombinant interferon alpha 2b in rheumatoid arthritis: good antigen for rheumatoid arthritis antibodies

WA Khan - Central European Journal of Immunology, 2018 - termedia.pl
recombinant interferon alpha 2b (riFn α-2b) in sera from different Ra patients. Utilization of
anti-hriFn α-2b antibodies as a probe for estimation of interferon α-2bEscherichia coli strain, …

Yeasts as Microbial Factories for Production of Recombinant Human Interferon Alpha 2b of Therapeutic Importance

S Katla, S Sivaprakasam - Next Generation Biomanufacturing …, 2019 - ACS Publications
… subtypes of IFNα2 are designated α-2a, α-2b, and α-2c (… (Escherichia coli) is the most
favored status for the production of … )with glucose as a carbon source for growth and huIFNα2b …

Exploring a combined Escherichia coli-based glycosylation and in vitro transglycosylation approach for expression of glycosylated interferon alpha

SK Prabhu, Q Yang, X Tong, LX Wang - Bioorganic & medicinal chemistry, 2021 - Elsevier
… Human interferon α-2b (IFNα) is used as a model protein to … kinetics likely affect the efficiency
of glucose transfer by ApNGT. … 6xHis-IFNα-Q158N recombinant protein as the acceptor …

Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality

E Karengera, A Robotham, J Kelly, Y Durocher… - Journal of …, 2017 - Elsevier
… We investigated the impact of glucose metabolism alteration by PYC2-overexpression on …
(lane 3) and 2 μg of non-glycosylated IFNα2b standard from Escherichia coli (lane 4). …

Recombinant interferon alpha-2b is a high-affinity antigen for type 1 diabetes autoantibodies

WA Khan - Canadian Journal of Diabetes, 2017 - Elsevier
… antibodies with recombinant interferon alpha-2b (hrIFN α-2b)… human interferon was cloned
and expressed in Escherichia coli. … For blood glucose estimations, blood was collected in …